glypican-1 Antibody (A-10): sc-365000

glypican-1 Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects glypican-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-glypican-1 antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glypican-1 (GPC1) is a crucial member of the glycosylphosphatidylinositol-anchored cell surface heparan sulfate proteoglycans, playing a significant role in cell adhesion, migration, and growth factor activity modulation. GPC1 interacts with fibroblast growth factors (FGFs), such as FGF-1, FGF-2, and FGF-7, which are essential for various cellular processes, including proliferation and differentiation. GPC1′s heparan sulfate chains facilitate binding to vascular endothelial growth factor 165 (VEGF165), acting as an extracellular chaperone that restores receptor binding ability after oxidative stress. This interaction supports hematopoietic stem and progenitor cell maintenance through GPC1 expression on marrow stromal cells. GPC1 shows elevated expression in human pancreatic cancer cells compared to normal pancreatic cells, suggesting involvement in tumor microenvironment and response to mitogenic stimuli like FGF-2. Human GPC1 gene location on chromosome 2q37.3 underscores genetic significance in various biological processes and disease states.

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glypican-1 Antibody (A-10) References:

1. Glypican-1 is a VEGF165 binding proteoglycan that acts as an extracellular chaperone for VEGF165.  |  Gengrinovitch, S., et al. 1999. J Biol Chem. 274: 10816-22. PMID: 10196157
2. Expression of proteoglycan core proteins in human bone marrow stroma.  |  Schofield, KP., et al. 1999. Biochem J. 343 Pt 3: 663-8. PMID: 10527946
3. Elevated glypican-1 expression is associated with an unfavorable prognosis in pancreatic ductal adenocarcinoma.  |  Lu, H., et al. 2017. Cancer Med. 6: 1181-1191. PMID: 28440066
4. A bispecific T cell engager targeting Glypican-1 redirects T cell cytolytic activity to kill prostate cancer cells.  |  Lund, ME., et al. 2020. BMC Cancer. 20: 1214. PMID: 33302918
5. Pharmacokinetics and Biodistribution of 89Zr-Miltuximab and Its Antibody Fragments as Glypican-1 Targeting Immuno-PET Agents in Glioblastoma.  |  Ghosh, S., et al. 2023. Mol Pharm. 20: 1549-1563. PMID: 36602058
6. Assignment of the human glypican gene (GPC1) to 2q35-q37 by fluorescence in situ hybridization.  |  Vermeesch, JR., et al. 1995. Genomics. 25: 327-9. PMID: 7774946
7. Integral membrane heparan sulfate proteoglycans.  |  David, G. 1993. FASEB J. 7: 1023-30. PMID: 8370471
8. Glypicans: a growing trend.  |  Weksberg, R., et al. 1996. Nat Genet. 12: 225-7. PMID: 8589707
9. Stimulation of fibroblast growth factor receptor-1 occupancy and signaling by cell surface-associated syndecans and glypican.  |  Steinfeld, R., et al. 1996. J Cell Biol. 133: 405-16. PMID: 8609172
10. Identification of glypican as a dual modulator of the biological activity of fibroblast growth factors.  |  Bonneh-Barkay, D., et al. 1997. J Biol Chem. 272: 12415-21. PMID: 9139688
11. The cell-surface heparan sulfate proteoglycan glypican-1 regulates growth factor action in pancreatic carcinoma cells and is overexpressed in human pancreatic cancer.  |  Kleeff, J., et al. 1998. J Clin Invest. 102: 1662-73. PMID: 9802880

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-glypican-1 Antibody (A-10) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-glypican-1 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

glypican-1 Antibody (A-10) Specifications

Alternate Product Name: glypican-1

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 71-165

Epitope Target: glypican-1

Epitope Species: human

Product Reactivity/Detection: glypican-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for glypican-1 Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin