Glyoxalase I Antibody (D-6): sc-133144

Glyoxalase I Antibody (D-6) is a mouse monoclonal IgG2a antibody that detects Glyoxalase I in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Glyoxalase I plays a crucial role in the detoxification of glucose degradation products, which is vital for cellular health and metabolic balance. This enzyme is particularly important in the context of oxidative stress, as GLO1 helps to mitigate the harmful effects of reactive carbonyl species that can arise from glucose metabolism. GLO1 exists in three isoforms, generated from two alleles in the genome, forming two homodimers and one heterodimer, with each subunit binding a zinc ion, which is essential for enzymatic activity. GLO1 expression has been shown to be induced in colon carcinoma, highlighting a potential role in cancer biology. Additionally, the presence of an insulin response element and a zinc metal response element in the promoter region of the GLO1 gene suggests that expression is tightly regulated by metabolic signals, making anti-Glyoxalase I antibody (D-6) a significant tool for research in both metabolic disorders and cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Glyoxalase I Antibody (D-6) References:

1. Glyoxalase I overexpression ameliorates renal ischemia-reperfusion injury in rats.  |  Kumagai, T., et al. 2009. Am J Physiol Renal Physiol. 296: F912-21. PMID: 19211689
2. Sugar beet M14 glyoxalase I gene can enhance plant tolerance to abiotic stresses.  |  Wu, C., et al. 2013. J Plant Res. 126: 415-25. PMID: 23203352
3. The temporal and spatial dynamics of glyoxalase I following excitoxicity and brain ischaemia.  |  Pieroh, P., et al. 2014. Biochem Soc Trans. 42: 534-7. PMID: 24646274
4. Piceatannol, a natural trans-stilbene compound, inhibits human glyoxalase I.  |  Takasawa, R., et al. 2017. Bioorg Med Chem Lett. 27: 1169-1174. PMID: 28169168
5. Expression of glyoxalase-I is reduced in cirrhotic livers: A possible mechanism in the development of cirrhosis.  |  Hollenbach, M., et al. 2017. PLoS One. 12: e0171260. PMID: 28231326
6. Multi-Armed 1,2,3-Selenadiazole and 1,2,3-Thiadiazole Benzene Derivatives as Novel Glyoxalase-I Inhibitors.  |  Al-Balas, QA., et al. 2019. Molecules. 24: PMID: 31487813
7. Polymorphisms in Glyoxalase I Gene Are Not Associated with Glyoxalase I Expression in Whole Blood or Markers of Methylglyoxal Stress: The CODAM Study.  |  Maasen, K., et al. 2021. Antioxidants (Basel). 10: PMID: 33540757
8. Glyoxalase I Assay as a Possible Tool for Evaluation of Biological Activity of Antioxidant-Rich Plant Extracts.  |  Laus, MN., et al. 2023. Plants (Basel). 12: PMID: 36904010
9. The PKM2 inhibitor shikonin enhances piceatannol-induced apoptosis of glyoxalase I-dependent cancer cells.  |  Inoue, M., et al. 2024. Genes Cells. 29: 52-62. PMID: 37963646
10. Novel anthraquinone amide derivatives as potential glyoxalase-I inhibitors.  |  Al-Akeedi, M., et al. 2024. J Med Life. 17: 87-98. PMID: 38737655

The preferred Glyoxalase I antibody is Glyoxalase I Antibody (D-5): sc-133214.