Glyoxalase I Antibody (D-5): sc-133214

Glyoxalase I Antibody (D-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Glyoxalase I (GLO1) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GLO1 antibody (D-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GLO1 plays a crucial role in the detoxification of glucose degradation products, which is vital for cellular health and metabolic balance. This enzyme catalyzes the conversion of methylglyoxal, a toxic byproduct of glycolysis, into less harmful substances, thereby protecting cells from oxidative stress and potential damage. GLO1′s importance is underscored by involvement in various physiological processes, including regulation of cellular responses to stress and potential link to anxiety disorders, as indicated by studies showing altered expression levels in mouse brains. GLO1 has three isoforms generated from two alleles, forming distinct homodimers and a heterodimer, with each subunit binding a zinc ion, which is essential for enzymatic activity. Research has also identified regulatory elements in GLO1 gene′s promoter region, including an insulin response element and a zinc metal response element, highlighting significance in metabolic regulation and cancer biology, particularly in colon carcinoma.

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Glyoxalase I Antibody (D-5) References:

1. Expression of glyoxalase I and II in normal and breast cancer tissues.  |  Rulli, A., et al. 2001. Breast Cancer Res Treat. 66: 67-72. PMID: 11368412
2. Catalytic mechanism of glyoxalase I: a theoretical study.  |  Himo, F. and Siegbahn, PE. 2001. J Am Chem Soc. 123: 10280-9. PMID: 11603978
3. Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor.  |  Junaid, MA., et al. 2004. Am J Med Genet A. 131: 11-7. PMID: 15386471
4. Identification of glyoxalase-I as a protein marker in a mouse model of extremes in trait anxiety.  |  Krömer, SA., et al. 2005. J Neurosci. 25: 4375-84. PMID: 15858064
5. Age-dependent changes of glyoxalase I expression in human brain.  |  Kuhla, B., et al. 2006. Neurobiol Aging. 27: 815-22. PMID: 15950319
6. Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione.  |  Yadav, SK., et al. 2005. Biochem Biophys Res Commun. 337: 61-7. PMID: 16176800
7. Association analysis of the functional Ala111Glu polymorphism of the glyoxalase I gene in panic disorder.  |  Politi, P., et al. 2006. Neurosci Lett. 396: 163-6. PMID: 16352396
8. Age- and stage-dependent glyoxalase I expression and its activity in normal and Alzheimer's disease brains.  |  Kuhla, B., et al. 2007. Neurobiol Aging. 28: 29-41. PMID: 16427160
9. Specificity of the trypanothione-dependent Leishmania major glyoxalase I: structure and biochemical comparison with the human enzyme.  |  Ariza, A., et al. 2006. Mol Microbiol. 59: 1239-48. PMID: 16430697
10. Crystallization and preliminary X-ray analysis of Leishmania major glyoxalase I.  |  Ariza, A., et al. 2005. Acta Crystallogr Sect F Struct Biol Cryst Commun. 61: 769-72. PMID: 16511153
11. Identification of the First 'Two Digit Nano-molar' Inhibitors of the Human Glyoxalase-I Enzyme as Potential Anticancer Agents.  |  Al-Balas, QA., et al. 2022. Med Chem. 18: 473-483. PMID: 34264188
12. Genome-Wide Identification of Cassava Glyoxalase I Genes and the Potential Function of MeGLYⅠ-13 in Iron Toxicity Tolerance.  |  Tang, F., et al. 2022. Int J Mol Sci. 23: PMID: 35563603

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glyoxalase I Antibody (D-5) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Glyoxalase I Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glyoxalase I Antibody (D-5) Specifications

Alternate Product Name: GLO1

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-184

Epitope Target: Glyoxalase I

Epitope Species: human

Product Reactivity/Detection: Glyoxalase I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Glyoxalase I Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin