Glycophorin A Antibody (R10): sc-53905

Glycophorin A Antibody (R10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Glycophorin A of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Glycophorin A antibody (R10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glycophorin A is a crucial sialoglycoprotein located on the surface of human erythrocytes, playing a significant role in maintaining red blood cell structural integrity and facilitating immune system interaction. Glycophorin A spans the membrane once, presenting an amino-terminal end to the extracellular environment, essential for blood group antigenicity function. The genetic diversity of glycophorin surface antigens, including Glycophorin A, B, and C, determines blood group phenotype, making these proteins vital for blood transfusion compatibility and understanding hemolytic diseases. The Glycophorin A gene, located on chromosome 4q31.21, consists of seven exons and exhibits high homology with Glycophorin B, encoding a 91 amino acid protein. Understanding Glycophorin A structure and function remains crucial for clinical applications and erythrocyte biology research, providing insights into blood group antigen expression and potential therapeutic targets for blood-related disorders.

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Glycophorin A Antibody (R10) References:

1. Immunohistochemical identification of erythroid precursors in paraffin embedded bone marrow sections: spectrin is a superior marker to glycophorin.  |  Sadahira, Y., et al. 1999. J Clin Pathol. 52: 919-21. PMID: 10711257
2. In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane.  |  Gerber, D. and Shai, Y. 2001. J Biol Chem. 276: 31229-32. PMID: 11402026
3. Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking.  |  Young, MT. and Tanner, MJ. 2003. J Biol Chem. 278: 32954-61. PMID: 12813056
4. Glycophorin A requirement for expression of O-linked antigens on the erythrocyte membrane.  |  Arimitsu, N., et al. 2003. Genes Cells. 8: 769-77. PMID: 12940824
5. Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A.  |  Bruce, LJ., et al. 2004. J Biol Chem. 279: 2414-20. PMID: 14604989
6. Structural adaptation of the glycophorin A transmembrane homodimer to D-amino acid modifications.  |  Gerber, D., et al. 2004. J Mol Biol. 339: 243-50. PMID: 15123435
7. Complex interactions at the helix-helix interface stabilize the glycophorin A transmembrane dimer.  |  Doura, AK. and Fleming, KG. 2004. J Mol Biol. 343: 1487-97. PMID: 15491626
8. Co-transfection of murine NXPE2 and murine glycophorin A confers reactivity with Ter-119.  |  Keele, GR., et al. 2024. Haematologica. 109: 3755-3759. PMID: 39021224
9. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia.  |  Andersson, LC., et al. 1979. Int J Cancer. 24: 717-20. PMID: 397196
10. Glycophorin A expression in malignant hematopoiesis.  |  Liszka, K., et al. 1983. Am J Hematol. 15: 219-26. PMID: 6638008
11. Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers.  |  Nakahata, T. and Okumura, N. 1994. Leuk Lymphoma. 13: 401-9. PMID: 8069185
12. Differentiation between bruises and putrefactive discolorations of the skin by immunological analysis of glycophorin A.  |  Kibayashi, K., et al. 1993. Forensic Sci Int. 61: 111-7. PMID: 8307520

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glycophorin A Antibody (R10) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Glycophorin A Antibody (R10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glycophorin A Antibody (R10) Specifications

Alternate Product Name: CD235a

Product Clone Name: R10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Glycophorin A

Epitope Species: human

Product Reactivity/Detection: Glycophorin A

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Glycophorin A Antibody (R10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin