Glycophorin A Antibody (JC159): sc-53295

Glycophorin A Antibody (JC159) is a mouse monoclonal IgG1 kappa light chain antibody that detects Glycophorin A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Glycophorin A antibody (JC159) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. Glycophorin A is a crucial sialoglycoprotein located in the erythrocyte membrane, where Glycophorin A plays a significant role in maintaining the structural integrity of red blood cells and facilitating cell-cell interactions. This sialoglycoprotein is essential for defining blood group phenotypes, as Glycophorin A bears the antigenic determinants for the MN blood group system. Glycophorin A on the erythrocyte surface not only contributes to the cell′s negative charge, which helps prevent aggregation, but also serves as a binding site for specific pathogens, influencing susceptibility to infections. The human Glycophorin A gene, located on chromosome 4q31.21, consists of seven exons and encodes a 150 amino acid protein that is 97% homologous to Glycophorin B, highlighting the evolutionary relationship between these proteins. Understanding Glycophorin A function and importance is vital for research in hematology and transfusion medicine, as variations in expression can have significant implications for blood compatibility and disease susceptibility.

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Glycophorin A Antibody (JC159) References:

1. In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane.  |  Gerber, D. and Shai, Y. 2001. J Biol Chem. 276: 31229-32. PMID: 11402026
2. SCL assembles a multifactorial complex that determines glycophorin A expression.  |  Lahlil, R., et al. 2004. Mol Cell Biol. 24: 1439-52. PMID: 14749362
3. Retrospective assessment of radiation exposure using biological dosimetry: chromosome painting, electron paramagnetic resonance and the glycophorin a mutation assay.  |  Kleinerman, RA., et al. 2006. Radiat Res. 166: 287-302. PMID: 16808614
4. A single mutation in the glycophorin A binding site of hepatitis A virus enhances virus clearance from the blood and results in a lower fitness variant.  |  Costafreda, MI., et al. 2012. J Virol. 86: 7887-95. PMID: 22593170
5. The glycophorin A transmembrane sequence within integrin αvβ3 creates a non-signaling integrin with low basal affinity that is strongly adhesive under force.  |  Müller, MA., et al. 2013. J Mol Biol. 425: 2988-3006. PMID: 23727145
6. Development and Characterization of Monoclonal Antibodies Against Glycophorin A Applicable for Blood Sample Processing.  |  Yang, J., et al. 2019. Monoclon Antib Immunodiagn Immunother. 38: 185-189. PMID: 31486711
7. The interaction between Glycophorin A (GPA) and Band 3 in the formation of the Wright b (Wrb) antigen.  |  Ekman, S., et al. 2021. Vox Sang. 116: 489-492. PMID: 33336813
8. Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure.  |  Siebert, PD. and Fukuda, M. 1986. Proc Natl Acad Sci U S A. 83: 1665-9. PMID: 3456608
9. Autoantibodies to red blood cell surface Glycophorin A impact the activation poise of circulating leukocytes.  |  Klein, MN., et al. 2022. Transfusion. 62: 217-226. PMID: 34796962
10. Effects of cholesterol and PIP2 on interactions between glycophorin A and Band 3 in lipid bilayers.  |  Qin, X., et al. 2022. Biophys J. 121: 2069-2077. PMID: 35524411

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glycophorin A Antibody (JC159) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Glycophorin A Antibody (JC159) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glycophorin A Antibody (JC159) Specifications

Alternate Product Name: Glycophorin A

Product Clone Name: JC159

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Glycophorin A

Epitope Species: human

Product Reactivity/Detection: Glycophorin A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Glycophorin A Antibody (JC159): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin

The preferred Glycophorin A antibody is Glycophorin A Antibody (R10): sc-53905.