Glycogenin-1 Antibody (E-11): sc-271109

Glycogenin-1 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Glycogenin-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Glycogenin-1 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glycogenin-1 plays a crucial role in glycogen synthesis, serving as a primer for glucose monomer addition in mammalian skeletal muscle. Glycogenin-1′s unique structure enables autoglucosylation, attaching glucose molecules to itself to initiate glycogen synthesis. The first glucose molecule from UDP-glucose binds to the hydroxyl group of tyrosine 194 on Glycogenin-1, and through glucosyltransferase activity, adds additional glucose residues until forming a chain of five. At this point, glycogen synthase takes over to further elongate the glycogen molecule. Glycogenin-1 remains covalently attached to the reducing end of glycogen, ensuring integral structure and function of this vital energy storage polysaccharide.

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Glycogenin-1 Antibody (E-11) References:

1. Characterization of mouse glycogenin-1 cDNA and promoter region.  |  van Maanen, M., et al. 1999. Biochim Biophys Acta. 1447: 284-90. PMID: 10542328
2. GNIP, a novel protein that binds and activates glycogenin, the self-glucosylating initiator of glycogen biosynthesis.  |  Skurat, AV., et al. 2002. J Biol Chem. 277: 19331-8. PMID: 11916970
3. De novo synthesis of bacterial glycogen: Agrobacterium tumefaciens glycogen synthase is involved in glucan initiation and elongation.  |  Ugalde, JE., et al. 2003. Proc Natl Acad Sci U S A. 100: 10659-63. PMID: 12960388
4. Creatine supplementation increases glycogen storage but not GLUT-4 expression in human skeletal muscle.  |  van Loon, LJ., et al. 2004. Clin Sci (Lond). 106: 99-106. PMID: 14507259
5. Glutaminyl cyclases unfold glutamyl cyclase activity under mild acid conditions.  |  Schilling, S., et al. 2004. FEBS Lett. 563: 191-6. PMID: 15063747
6. Glycogenin: the primer for mammalian and yeast glycogen synthesis.  |  Lomako, J., et al. 2004. Biochim Biophys Acta. 1673: 45-55. PMID: 15238248
7. The C-terminal residues in the alpha-interacting domain (AID) helix anchor CaV beta subunit interaction and modulation of CaV2.3 channels.  |  Berrou, L., et al. 2005. J Biol Chem. 280: 494-505. PMID: 15507442
8. Glycogenin is the priming glucosyltransferase required for the initiation of glycogen biogenesis in rabbit skeletal muscle.  |  Pitcher, J., et al. 1988. Eur J Biochem. 176: 391-5. PMID: 2970965

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glycogenin-1 Antibody (E-11) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Glycogenin-1 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glycogenin-1 Antibody (E-11) Specifications

Alternate Product Name: Glycogenin-1

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 311-350

Epitope Target: Glycogenin-1

Epitope Species: human

Product Reactivity/Detection: Glycogenin-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Glycogenin-1 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin