GluRS Antibody (G-1): sc-271728

GluRS Antibody (G-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects GluRS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GluRS antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GluRS, or glutamyl-tRNA synthetase 2, is a crucial enzyme that plays a significant role in protein biosynthesis by catalyzing the attachment of glutamate to its corresponding tRNA, a process essential for accurate translation of genetic information into proteins. This enzyme operates within the mitochondrial matrix, where GluRS ensures the fidelity of protein synthesis by linking amino acids to their respective tRNA molecules, thus facilitating the correct incorporation of glutamate into polypeptide chains. The two-step reaction involves the ATP-dependent activation of glutamate to form Glu-AMP, followed by the transfer of the glutamate residue to tRNA(Glu). Proper functioning of GluRS is vital, as errors in aminoacylation can lead to the production of malfunctioning proteins, which may contribute to various diseases, including mitochondrial disorders.

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GluRS Antibody (G-1) References:

1. Macromolecular assemblage of aminoacyl-tRNA synthetases: quantitative analysis of protein-protein interactions and mechanism of complex assembly.  |  Robinson, JC., et al. 2000. J Mol Biol. 304: 983-94. PMID: 11124041
2. Molecular network and functional implications of macromolecular tRNA synthetase complex.  |  Han, JM., et al. 2003. Biochem Biophys Res Commun. 303: 985-93. PMID: 12684031
3. Aminoacyl-tRNA synthetase complexes: beyond translation.  |  Lee, SW., et al. 2004. J Cell Sci. 117: 3725-34. PMID: 15286174
4. Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.  |  Bonnefond, L., et al. 2005. Biochemistry. 44: 4805-16. PMID: 15779907
5. Dynamical networks in tRNA:protein complexes.  |  Sethi, A., et al. 2009. Proc Natl Acad Sci U S A. 106: 6620-5. PMID: 19351898
6. Identity switches between tRNAs aminoacylated by class I glutaminyl- and class II aspartyl-tRNA synthetases.  |  Frugier, M., et al. 1994. Biochemistry. 33: 9912-21. PMID: 8060999
7. Glutamyl-tRNA sythetase.  |  Freist, W., et al. 1997. Biol Chem. 378: 1313-29. PMID: 9426192
8. Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein.  |  Quevillon, S., et al. 1999. J Mol Biol. 285: 183-95. PMID: 9878398

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GluRS Antibody (G-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-GluRS Antibody (G-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GluRS Antibody (G-1) Specifications

Alternate Product Name: EARS2

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: GluRS

Epitope Species: human

Product Reactivity/Detection: GluRS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for GluRS Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin