GLUD1/2 Antibody (C-10): sc-515542
- GLUD1/2 Antibody (C-10) is a mouse monoclonal IgG1 κ GLUD1/2 antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of GLUD1 of human origin
- GLUD1/2 Antibody (C-10) is recommended for detection of GLUD1 and GLUD2 of human origin, and GLUD1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-GLUD1/2 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GLUD1/2 Antibody (C-10) for WB and IHC(P) applications. These reagents are now offered in bundles with GLUD1/2 Antibody (C-10) (see ordering information below).
GLUD1/2 (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects GLUD1/2 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). GLUD1/2 (C-10) antibody is available as non-conjugated form and multiple conjugates including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and various Alexa Fluor® forms. GLUD1 (glutamate dehydrogenase 1), also known as GDH, GDH1 or GLUD, and GLUD2 (glutamate dehydrogenase 2), also known as GDH2 or GLUDP1, are mitochondrial matrix enzymes belonging to the Glu/Leu/Phe/Val dehydrogenases family. Existing as homohexamers, GLUD1 catalyzes the oxidative deamination of glutamate to α-ketoglutarate and ammonia while GLUD2 is involved in glutamate recycling during neurotransmission. GLUD1 regulates amino acid induced insulin secretion and is allosterically activated by ADP and inhibited by GTP and ATP. Mutations in GLUD1 cause hyperinsulinism-hyperammonemia syndrome (HHS), an inherited condition characterized by high insulin and ammonia levels in blood. GLUD1 may contribute to learning and memory by increasing glutamate turnover. GLUD2 is expressed primarily in testis and retina, with lower expression in brain.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GLUD1/2 Antibody (C-10) References:
- Molecular basis and characterization of the hyperinsulinism/hyperammonemia syndrome: predominance of mutations in exons 11 and 12 of the glutamate dehydrogenase gene. HI/HA Contributing Investigators. | Stanley, CA., et al. 2000. Diabetes. 49: 667-73. PMID: 10871207
- Unregulated elevation of glutamate dehydrogenase activity induces glutamine-stimulated insulin secretion: identification and characterization of a GLUD1 gene mutation and insulin secretion studies with MIN6 cells overexpressing the mutant glutamate dehydrogenase. | Tanizawa, Y., et al. 2002. Diabetes. 51: 712-7. PMID: 11872671
- Advances in the diagnosis and management of hyperinsulinemic hypoglycemia. | Kapoor, RR., et al. 2009. Nat Clin Pract Endocrinol Metab. 5: 101-12. PMID: 19165222
- Mutations in human GLUD2 glutamate dehydrogenase affecting basal activity and regulation. | Kanavouras, K., et al. 2009. J Neurochem. 109 Suppl 1: 167-73. PMID: 19393024
- The human GLUD2 glutamate dehydrogenase: localization and functional aspects. | Zaganas, I., et al. 2009. Neurochem Int. 55: 52-63. PMID: 19428807
- Human GLUD1 and GLUD2 glutamate dehydrogenase localize to mitochondria and endoplasmic reticulum. | Mastorodemos, V., et al. 2009. Biochem Cell Biol. 87: 505-16. PMID: 19448744
- Glutamate dehydrogenase activity in lymphocytes of B-cell chronic lymphocytic leukaemia patients. | Pajic, T., et al. 2009. Clin Biochem. 42: 1677-84. PMID: 19683518
- Hyperinsulinism-hyperammonaemia syndrome: novel mutations in the GLUD1 gene and genotype-phenotype correlations. | Kapoor, RR., et al. 2009. Eur J Endocrinol. 161: 731-5. PMID: 19690084
- Gain-of-function variant in GLUD2 glutamate dehydrogenase modifies Parkinson's disease onset. | Plaitakis, A., et al. 2010. Eur J Hum Genet. 18: 336-41. PMID: 19826450
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLUD1/2 Antibody (C-10) | sc-515542 | 200 µg/ml | $316.00 | |||
GLUD1/2 Antibody (C-10): m-IgG Fc BP-HRP Bundle | sc-531405 | 200 µg Ab; 10 µg BP | $354.00 | |||
GLUD1/2 Antibody (C-10): m-IgGκ BP-HRP Bundle | sc-525088 | 200 µg Ab, 40 µg BP | $354.00 | |||
GLUD1/2 Antibody (C-10): m-IgG1 BP-HRP Bundle | sc-544533 | 200 µg Ab; 20 µg BP | $354.00 | |||
GLUD1/2 Antibody (C-10) AC | sc-515542 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GLUD1/2 Antibody (C-10) HRP | sc-515542 HRP | 200 µg/ml | $316.00 | |||
GLUD1/2 Antibody (C-10) FITC | sc-515542 FITC | 200 µg/ml | $330.00 | |||
GLUD1/2 Antibody (C-10) PE | sc-515542 PE | 200 µg/ml | $343.00 | |||
GLUD1/2 Antibody (C-10) Alexa Fluor® 488 | sc-515542 AF488 | 200 µg/ml | $357.00 | |||
GLUD1/2 Antibody (C-10) Alexa Fluor® 546 | sc-515542 AF546 | 200 µg/ml | $357.00 | |||
GLUD1/2 Antibody (C-10) Alexa Fluor® 594 | sc-515542 AF594 | 200 µg/ml | $357.00 | |||
GLUD1/2 Antibody (C-10) Alexa Fluor® 647 | sc-515542 AF647 | 200 µg/ml | $357.00 | |||
GLUD1/2 Antibody (C-10) Alexa Fluor® 680 | sc-515542 AF680 | 200 µg/ml | $357.00 | |||
GLUD1/2 Antibody (C-10) Alexa Fluor® 790 | sc-515542 AF790 | 200 µg/ml | $357.00 |