Glucosidase I Antibody (C-11): sc-374006
- Glucosidase I Antibody (C-11) is a mouse monoclonal IgG2b κ Glucosidase I antibody provided at 200 µg/ml
- raised against a peptide mapping near the N-terminus of Glucosidase I of human origin
- Glucosidase I Antibody (C-11) is recommended for detection of Glucosidase I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Glucosidase I Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Glucosidase I Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Glucosidase I Antibody (C-11) (see ordering information below).
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Glucosidase I Antibody (C-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Glucosidase I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Glucosidase I (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glucosidase I plays a crucial role in the N-linked glycosylation pathway, specifically catalyzing the removal of the terminal alpha 1,2-linked glucose residue from the Glc3-Man9-GlcNAc2 oligosaccharide precursor in the endoplasmic reticulum. This process is vital for proper protein folding and function, as glycosylation is essential for the stability and activity of many proteins. Glucosidase I functions within the lumen of the endoplasmic reticulum, where Glucosidase I interacts with nascent glycoproteins, ensuring correct modification before transport to final destinations. Mutations in the gene encoding Glucosidase I can lead to congenital disorders of glycosylation, such as CDG-IIb, which presents with severe clinical manifestations including hypotonia, dysmorphic features, and neurological complications. Understanding Glucosidase I function and importance is critical for elucidating the mechanisms underlying these disorders and developing potential therapeutic strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Glucosidase I Antibody (C-11) References:
- Genomic organization and promoter activity of glucosidase I gene. | Khan, FA., et al. 1999. Glycobiology. 9: 797-806. PMID: 10406845
- A novel disorder caused by defective biosynthesis of N-linked oligosaccharides due to glucosidase I deficiency. | De Praeter CM, ., et al. 2000. Am J Hum Genet. 66: 1744-56. PMID: 10788335
- Processing of N-linked carbohydrate chains in a patient with glucosidase I deficiency (CDG type IIb). | Völker, C., et al. 2002. Glycobiology. 12: 473-83. PMID: 12145188
- (Arg)3 within the N-terminal domain of glucosidase I contains ER targeting information but is not required absolutely for ER localization. | Hardt, B., et al. 2003. Glycobiology. 13: 159-68. PMID: 12626409
- The Lec23 Chinese hamster ovary mutant is a sensitive host for detecting mutations in alpha-glucosidase I that give rise to congenital disorder of glycosylation IIb (CDG IIb). | Hong, Y., et al. 2004. J Biol Chem. 279: 49894-901. PMID: 15383536
- N-glycan processing in ER quality control. | Ruddock, LW. and Molinari, M. 2006. J Cell Sci. 119: 4373-80. PMID: 17074831
- Cloning and expression of glucosidase I from human hippocampus. | Kalz-Füller, B., et al. 1995. Eur J Biochem. 231: 344-51. PMID: 7635146
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucosidase I Antibody (C-11) | sc-374006 | 200 µg/ml | $316.00 | |||
Glucosidase I Antibody (C-11): m-IgGκ BP-HRP Bundle | sc-522586 | 200 µg Ab, 40 µg BP | $354.00 | |||
Glucosidase I Antibody (C-11): m-IgG2b BP-HRP Bundle | sc-549044 | 200 µg Ab; 10 µg BP | $354.00 | |||
Glucosidase I Antibody (C-11) AC | sc-374006 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Glucosidase I Antibody (C-11) HRP | sc-374006 HRP | 200 µg/ml | $316.00 | |||
Glucosidase I Antibody (C-11) FITC | sc-374006 FITC | 200 µg/ml | $330.00 | |||
Glucosidase I Antibody (C-11) PE | sc-374006 PE | 200 µg/ml | $343.00 | |||
Glucosidase I Antibody (C-11) Alexa Fluor® 488 | sc-374006 AF488 | 200 µg/ml | $357.00 | |||
Glucosidase I Antibody (C-11) Alexa Fluor® 546 | sc-374006 AF546 | 200 µg/ml | $357.00 | |||
Glucosidase I Antibody (C-11) Alexa Fluor® 594 | sc-374006 AF594 | 200 µg/ml | $357.00 | |||
Glucosidase I Antibody (C-11) Alexa Fluor® 647 | sc-374006 AF647 | 200 µg/ml | $357.00 | |||
Glucosidase I Antibody (C-11) Alexa Fluor® 680 | sc-374006 AF680 | 200 µg/ml | $357.00 | |||
Glucosidase I Antibody (C-11) Alexa Fluor® 790 | sc-374006 AF790 | 200 µg/ml | $357.00 |