Glucosidase I Antibody (C-11) recommended for detection of Glucosidase I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Glucosidase I Antibody (C-11): sc-374006

Glucosidase I Antibody (C-11) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-Glucosidase I Antibody (C-11) is a mouse monoclonal IgG2b (kappa light chain) Glucosidase I antibody provided at 200 µg/ml
    • raised against a peptide mapping near the N-terminus of Glucosidase I of human origin
    • Anti-Glucosidase I Antibody (C-11) is recommended for detection of Glucosidase I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Glucosidase I Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Glucosidase I (C-11): sc-374006.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Glucosidase I Antibody (C-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with Glucosidase I Antibody (C-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can Glucosidase I (C-11): sc-374006 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, Glucosidase I (C-11): sc-374006 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-13
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