Date published: 2025-11-21

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Glucagon Antibody (C-11): sc-514592

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Datasheets
  • Glucagon Antibody (C-11) is a mouse monoclonal IgG1 κ, cited in 42 publications, provided at 200 µg/ml
  • raised against amino acids 1-180 representing full length Glucagon of human origin
  • Anti-Glucagon Antibody (C-11) is recommended for detection of Proglucagon, Glucagon, GLP-1 and GLP-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-Glucagon Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Glucagon Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Glucagon Antibody (C-11) (see ordering information below).

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Glucagon Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Glucagon in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Glucagon antibody (C-11) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Glucagon is a crucial pancreatic hormone that plays a vital role in glucose metabolism by acting as an antagonist to insulin; Glucagon stimulates the conversion of glycogen to glucose, thereby increasing blood sugar levels, which is essential for maintaining energy homeostasis in the body. Glucagon is primarily located in the alpha cells of the pancreas, where Glucagon is synthesized from proglucagon. This localization enables rapid release of Glucagon in response to low blood glucose levels, ensuring quick mobilization of energy reserves when needed. Glucagon′s interaction with receptors in the liver and other tissues is critical for glucose regulation, making Glucagon a key player in metabolic processes and a target for therapeutic interventions in conditions such as diabetes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Glucagon Antibody (C-11)

sc-514592
200 µg/ml
$316.00

Glucagon Antibody (C-11): m-IgG Fc BP-HRP Bundle

sc-527919
200 µg Ab; 10 µg BP
$354.00

Glucagon Antibody (C-11): m-IgG1 BP-HRP Bundle

sc-533292
200 µg Ab; 20 µg BP
$354.00

Glucagon Antibody (C-11) AC

sc-514592 AC
500 µg/ml, 25% agarose
$416.00

Glucagon Antibody (C-11) HRP

sc-514592 HRP
200 µg/ml
$316.00

Glucagon Antibody (C-11) FITC

sc-514592 FITC
200 µg/ml
$330.00

Glucagon Antibody (C-11) PE

sc-514592 PE
200 µg/ml
$343.00

Glucagon Antibody (C-11) Alexa Fluor® 488

sc-514592 AF488
200 µg/ml
$357.00

Glucagon Antibody (C-11) Alexa Fluor® 546

sc-514592 AF546
200 µg/ml
$357.00

Glucagon Antibody (C-11) Alexa Fluor® 594

sc-514592 AF594
200 µg/ml
$357.00

Glucagon Antibody (C-11) Alexa Fluor® 647

sc-514592 AF647
200 µg/ml
$357.00

Glucagon Antibody (C-11) Alexa Fluor® 680

sc-514592 AF680
200 µg/ml
$357.00

Glucagon Antibody (C-11) Alexa Fluor® 790

sc-514592 AF790
200 µg/ml
$357.00

Hello, can this antibody detect the GLP-1 in mouse tissue by immunofluorescent?

Asked by: Hong
Thank you for your question. Anti-Glucagon Antibody (C-11) is recommended for detection of Proglucagon, Glucagon, GLP-1 and GLP-2 of mouse, rat and human origin by Immunofluorescence, WB, IP, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2025-05-26

Glucagon Antibody (C-11) HRP, sc-514592 HRP 200 µg/ml: Would this antibody reacts with liraglutide (HAEGTFTSDVSSYLEGQAAKEFIAWLVRGRG) in western-blotting.

Asked by: Pavan
Thank you for your question. Glucagon Antibody (C-11) HRP is a mouse monoclonal IgG raised against amino acids 1-180 of Glucagon of human origin. The exact epitope recognized by this antibody has not been determined, so although this aligns 100% with the liraglutide sequence you provided above, since we do not know exactly where the epitope is within 1-180, we can not guarantee that it can pick up the peptide in a WB experiment. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-03-17

Can Glucagon (C-11): sc-514592 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Glucagon (C-11): sc-514592 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2021-08-23

Hi there. I am wondering whether you can tell me the specific IgG isotype of this antibody: Glucagon Antibody (C-11): sc-514592. Thank you!

Asked by: Juliaia
Thank you for your question. This antibody, sc-514592: Glucagon (C-11) is a mouse monoclonal IgG1 (kappa light chain).
Answered by: Technical Support
Date published: 2016-11-15
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Rated 3 out of 5 by from 试用装的Glucagon 抗体量明显不足10ug,应该只有6ul,影响使用体验感,希望改善试用装的Glucagon 抗体量明显不足10ug,应该只有6ul,用的比例是1:50,做出来了,但是试用装量与描述不符,影响使用体验感,希望改善
Date published: 2024-07-02
Rated 5 out of 5 by from Veramente ottimo (testato su tessuto di gatto)L'anticorpo è ottimo anche per immunoistochimica su tessuto pancreatico di gatto.
Date published: 2024-01-11
Rated 5 out of 5 by from Great antibody for IFStrong and clear signal on mouse pancreatic FFPE sections at 1/300 incubated ON 4°C and detected by Alexa 594.
Date published: 2018-01-05
Rated 5 out of 5 by from excellent antibody for immunofluorescent stainingTest it on mouse pancreatic tissue cryosections (1:100) and counterstain with DAPI.
Date published: 2017-12-07
Rated 5 out of 5 by from worked wellI brought this one month ago,it WORK well WITH IF.
Date published: 2017-10-23
Rated 5 out of 5 by from Excellent AntibodyWith concentration about 0.5ug/ml, weget perfect result!
Date published: 2017-07-25
Rated 5 out of 5 by from Worked well on FFPE mouse tissueTested antibody on FFPE paraffin sections of mouse pancreas with following conditions: HIER 20min @ 100C pH6 Blocked with Biocare Rodent Block M - 20min RT Incubated ON @ 4C with sc-514592 @ 1:100 Detected with Alexa 647 and counterstained with DAPI
Date published: 2017-03-13
Rated 5 out of 5 by from Good with FFPEThis antibody provides strong staining in IHC with paraffin-embedded sections when treated with citrate buffer and heat for antigen retrieval.
Date published: 2017-02-07
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Glucagon Antibody (C-11) is rated 4.8 out of 5 by 9.
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