Glucagon Antibody (K79bB10) recommended for detection of Glucagon of mouse, rat, human, porcine, feline and canine origin by WB, IP, IF and IHC(P)

Glucagon Antibody (K79bB10): sc-57171

Glucagon Antibody (K79bB10) is rated 4.0 out of 5 by 2.
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Datasheets

    • Glucagon Antibody (K79bB10) is a mouse monoclonal IgG1 provided at 200 µg/ml
    • raised against polymerized Glucagon of porcine origin
    • Anti-Glucagon Antibody (K79bB10) is recommended for detection of Glucagon of mouse, rat, human, porcine, feline and canine origin by WB, IP, IF and IHC(P); not recommended for detection of enteroglucagon
    • Anti-Glucagon Antibody (K79bB10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Glucagon (K79bB10): sc-57171.
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Is this product corresponding to internal sequence amino acids 53-81 of Human Glucagon?

Asked by: cseunglim
Thank you for your question. Unfortunately this antibody has not been epitope mapped, so we do not have this information. The antibody was raised against polymerized porcine glucagon. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-21

I am using Glucagon (K79bB10): sc-57171 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-27
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Rated 5 out of 5 by from Highly recommended for IHC(P) Great Cytoplasmic Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue. Also reported to be cross reactive with Cat, Dog, Guniea pig, and Rabbit
Date published: 2016-12-29
Rated 3 out of 5 by from Good immunoperoxidase cytoplasmic staining Good immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2013-10-28
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