Date published: 2025-9-5
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GLI-1/GLI1 Antibody (C-1) - Western Blotting - Image 363219
GLI-1 抗体 (C-1): sc-515751. SK-N-MC (A), U-87 MG (B), Saos-2 (C), PC-3 (D) 和 NTERA-2 cl. D1 (E) 全细胞裂解液及 K-562 核提取物 (F) 中 GLI-1 表达的 Western 印迹分析.
GLI-1 抗体 (C-1): sc-515751. SK-N-MC (A), U-87 MG (B), Saos-2 (C), PC-3 (D) 和 NTERA-2 cl. D1 (E) 全细胞裂解液及 K-562 核提取物 (F) 中 GLI-1 表达的 Western 印迹分析.

GLI-1/GLI1 抗体 (C-1): sc-515751

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寫評論提問

说明书
  • GLI-1/GLI1抗体(C-1)是小鼠单克隆IgG1 κ, 在38篇文献中引用，规格为200 µg/ml
  • 免疫human物种的的氨基酸781-1080
  • 抗-GLI-1/GLI1 抗体 (C-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 GLI-1
  • 抗GLI-1/GLI1抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-515751 X, 200 µg/0.1 ml)
  • 关于如何获取GLI-1/GLI1 (C-1): sc-515751的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是GLI-1/GLI1 Antibody (C-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与GLI-1/GLI1 Antibody (C-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外GLI抗体，包括GLI-1, GLI-2和GLI-3

GLI-1抗体（C-1）是一种小鼠单克隆IgG1 kappa轻链抗体，经过工程改造，能够识别人类GLI-1（又称胶质瘤相关致癌基因或致癌基因GLI）。抗GLI-1抗体（C-1）针对全长GLI-1蛋白的氨基酸781-1080，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）在小鼠、大鼠和人类样本中进行有效检测。GLI-1是锌指转录因子GLI家族的一员，在Hedgehog信号通路中发挥着关键作用，该通路对胚胎发育、组织形态和干细胞群的维持至关重要。GLI-1主要位于细胞核中，通过与特定DNA序列结合并调节细胞增殖、分化及存活相关靶基因的表达，发挥转录激活剂的功能。GLI-1的核定位对于调节基因表达至关重要，而基因表达失调与包括胶质瘤在内的多种癌症有关，这凸显了GLI-1在正常生理和致癌过程中的重要性。GLI-1与其他蛋白质（如SUFU）和各种协同激活因子相互作用，以微调转录活性，确保精确控制细胞对发育和环境信号的反应。抗GLI-1抗体（C-1）是研究Hedgehog信号传导机制、癌症生物学和发育生物学的研究人员不可或缺的工具，为这一关键转录因子的调控和功能提供了宝贵的见解。GLI-1单克隆抗体（C-1）有未结合和结合两种形式，为各种实验应用提供了多功能性，有助于对GLI-1所调控的分子通路进行深入研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GLI-1/GLI1 抗体 (C-1) 参考文献:

  1. 融合和抑制融合的对立活动对 Gli 的调控。  |  Murone, M., et al. 2000. Nat Cell Biol. 2: 310-2. PMID: 10806483
  2. 致编辑的信：GLI1癌基因多态变异的鉴定。  |  Wang, XQ., et al. 2000. J Invest Dermatol. 115: 328-9. PMID: 10951255
  3. Zic 蛋白和 Gli 蛋白之间的物理和功能相互作用。  |  Koyabu, Y., et al. 2001. J Biol Chem. 276: 6889-92. PMID: 11238441
  4. 人类锌指蛋白家族，可结合甲基化 DNA 并抑制转录。  |  Filion, GJ., et al. 2006. Mol Cell Biol. 26: 169-81. PMID: 16354688
  5. 人类 KRAB 相关锌指基因综合目录：深入了解转录抑制因子大家族的进化史。  |  Huntley, S., et al. 2006. Genome Res. 16: 669-77. PMID: 16606702
  6. [KRAB 锌指蛋白研究进展]。  |  Tian, CY., et al. 2006. Yi Chuan. 28: 1451-6. PMID: 17098717
  7. Gli-1 siRNA诱导Huh7细胞凋亡。  |  Chen, XL., et al. 2008. World J Gastroenterol. 14: 582-9. PMID: 18203291
  8. 一种促进胶质母细胞瘤细胞迁移和侵袭的新型 GLI1 剪接变体。  |  Lo, HW., et al. 2009. Cancer Res. 69: 6790-8. PMID: 19706761
  9. GLI 基因是锌指蛋白 Kruppel 家族的成员。  |  Kinzler, KW., et al. 1988. Nature. 332: 371-4. PMID: 2832761
  10. 上皮牙源性病变中SHH、SMO和GLI-1蛋白的免疫组织化学分析。  |  Rodrigues, KS., et al. 2022. Braz Dent J. 33: 91-99. PMID: 36287504
  11. 淫羊藿素通过上调GLI-1，促进骨质疏松症和T2DM患者骨源性间充质干细胞的增殖和成骨分化。  |  Xia, SL., et al. 2023. J Orthop Surg Res. 18: 500. PMID: 37454090
  12. 五指 GLI-DNA 复合物的晶体结构：锌指的新视角。  |  Pavletich, NP. and Pabo, CO. 1993. Science. 261: 1701-7. PMID: 8378770

订购信息

产品名称产品编号规格价格数量收藏夹

GLI-1/GLI1 抗体 (C-1)

sc-515751
200 µg/ml
$316.00

GLI-1/GLI1 (C-1): m-IgG Fc BP-HRP 套装

sc-531496
200 µg Ab; 10 µg BP
$354.00

GLI-1/GLI1 (C-1): m-IgGκ BP-HRP 套装

sc-525195
200 µg Ab, 40 µg BP
$354.00

GLI-1/GLI1 (C-1): m-IgG1 BP-HRP 套装

sc-544591
200 µg Ab; 20 µg BP
$354.00

GLI-1/GLI1 抗体 (C-1) X

sc-515751 X
200 µg/0.1 ml
$316.00

GLI-1/GLI1 抗体 (C-1) AC

sc-515751 AC
500 µg/ml, 25% agarose
$416.00

GLI-1/GLI1 抗体 (C-1) HRP

sc-515751 HRP
200 µg/ml
$316.00

GLI-1/GLI1 抗体 (C-1) FITC

sc-515751 FITC
200 µg/ml
$330.00

GLI-1/GLI1 抗体 (C-1) PE

sc-515751 PE
200 µg/ml
$343.00

GLI-1/GLI1 抗体 (C-1) Alexa Fluor® 488

sc-515751 AF488
200 µg/ml
$357.00

GLI-1/GLI1 抗体 (C-1) Alexa Fluor® 546

sc-515751 AF546
200 µg/ml
$357.00

GLI-1/GLI1 抗体 (C-1) Alexa Fluor® 594

sc-515751 AF594
200 µg/ml
$357.00

GLI-1/GLI1 抗体 (C-1) Alexa Fluor® 647

sc-515751 AF647
200 µg/ml
$357.00

GLI-1/GLI1 抗体 (C-1) Alexa Fluor® 680

sc-515751 AF680
200 µg/ml
$357.00

GLI-1/GLI1 抗体 (C-1) Alexa Fluor® 790

sc-515751 AF790
200 µg/ml
$357.00

你好，这个抗体可以做Chop-qPCR吗

Asked by: Zzzzzziiiii
感谢您的提问。该抗体不推荐做Chop-qPCR。
Answered by: Technical Service SH
Date published: 2024-07-08

Has it been tested for human IHC(P) yet? If it isn't, is there any Santa Cruz anti-Gli1 validated for IHC(P)? I have searched for the antibody you recommended two years ago but it seems it's not available anymore. Thank you very much

Asked by: Pablo19
Thank you for your question. We have not yet tested this antibody on formalin-fixed paraffin-embedded tissue sections and so IHC(P) is not a recommended application at this time. We do currently offer an alternative antibody that has been tested for human IHC(P), GLI-1 Antibody (A-7): sc-515781.
Answered by: Technical Support
Date published: 2019-02-27

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515751: GLI-1 (C-1) mouse monoclonal antibody will work?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06

Are they good for human IHC(P)? Thank you.

Asked by: Natalia11
Thank you for your question. We have not yet tested our, GLI-1 Antibody (C-1): sc-515751, on formalin-fixed paraffin-embedded tissue sections, so we do not know if it is suitable for IHC(P). We do currently warranty the antibody to work for IF, or immunohistochemistry or IF on fresh/frozen tissues/cells fixed in methanol or acetone. We do offer an alternative antibody to GLI-1 that is validated to work for IHC(P) on human tissues, GLI-1 Antibody (1B9F8): sc-517290 (https://www.scbt.com/scbt/product/gli-1-antibody-1b9f8)
Answered by: Technical Support
Date published: 2017-02-03
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Rated 5 out of 5 by from good signalWe used this antibody to investigate the expression and clinical significance of Gli1 protein in breast cancer tissues in Hedgehog signaling pathway,we found this antibody is very useful ,we got strong signal around 135kda use the dilution 1:800.
Date published: 2017-08-12
Rated 5 out of 5 by from Good antibodyUsing the concentration at 1:800/1:1000 for western blot is very good,
Date published: 2017-08-09
Rated 5 out of 5 by from very good with WBWe got the sample for this antibody and it worked excellent for Western Blot! Strong Signal and clean background! We used human lung cancer cell lines PC9 A549 and H1299.
Date published: 2017-07-06
Rated 5 out of 5 by from great qualityWorks very well for western blotting; strong signal with no background; great antibody.
Date published: 2017-05-27
Rated 5 out of 5 by from Best one we've used.We've used other Gli1 antibodies with mediocre results- inconsistent banding, short degradation time of the antibody, poor signal. This Gli1, with the corresponding m-IgGk BP-HRP secondary gave us great and repeatable Western Blot results on a sometimes tricky project. See the basic WB data below where a tagged Gli1 was over expressed in a cell line (first 3 lanes) and empty vector controls (last 3 lines).
Date published: 2017-03-16
Rated 5 out of 5 by from the function of this antibody is very goodI had tried Gli-1 antibody that comes from other company before I tried this antibody. I found that this antibody is very perfect
Date published: 2017-02-28
Rated 5 out of 5 by from no complaintsI use this antibody do western blot at 1:500 dilution, in the F9 whole cell lysate the band is specific.
Date published: 2017-02-12
Rated 3 out of 5 by from oxygenation reactions.It is well known that zinc finger proteins include some form of transcriptional activation or inhibition that interacts with oxidases in the human body. Our experiments are performed by recruiting histone-modified proteins for oxygenation reactions.
Date published: 2017-02-12
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GLI-1/GLI1 抗体 (C-1) is rated 4.3 out of 5 by 12.
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