GLCNE Antibody (H-10): sc-376057

GLCNE Antibody (H-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects GLCNE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GLCNE (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GLCNE protein, also known as UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, plays a crucial role in the biosynthesis of N-acetylneuraminic acid (NeuAc), which is a key precursor for sialic acids. Sialic acids are essential for various biological processes, including cell adhesion, signal transduction, and the modulation of tumorigenic and metastatic behaviors in malignant cells. GLCNE is particularly important for normal sialylation in hematopoietic cells, and GLCNE expression is significantly upregulated following protein kinase C-dependent phosphorylation. GLCNE is predominantly found in the liver and placenta, with lower levels detected in the heart, brain, lung, kidney, skeletal muscle, and pancreas. Mutations or defects in GLCNE are associated with several disorders, including sialuria, inclusion body myopathy 2 (IBM2), and Nonaka myopathy (NM), which are characterized by various neuromuscular symptoms and metabolic dysregulation. Sialuria results from a lack of feedback inhibition by CMP-NeuAc, leading to an overproduction of NeuAc and subsequent accumulation in the cytoplasm and urine. Anti-GLCNE antibody (H-10) is an invaluable tool for researchers studying the functional implications of GLCNE in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GLCNE Antibody (H-10) References:

1. No overall hyposialylation in hereditary inclusion body myopathy myoblasts carrying the homozygous M712T GNE mutation.  |  Salama, I., et al. 2005. Biochem Biophys Res Commun. 328: 221-6. PMID: 15670773
2. Distal myopathy with rimmed vacuoles and hereditary inclusion body myopathy.  |  Nonaka, I., et al. 2005. Curr Neurol Neurosci Rep. 5: 61-5. PMID: 15676110
3. Localization of UDP-GlcNAc 2-epimerase/ManAc kinase (GNE) in the Golgi complex and the nucleus of mammalian cells.  |  Krause, S., et al. 2005. Exp Cell Res. 304: 365-79. PMID: 15748884
4. Allelic heterogeneity of GNE gene mutation in two Tunisian families with autosomal recessive inclusion body myopathy.  |  Amouri, R., et al. 2005. Neuromuscul Disord. 15: 361-3. PMID: 15833430
5. Phenotypic variability in a Chinese family with rimmed vacuolar distal myopathy.  |  Ro, LS., et al. 2005. J Neurol Neurosurg Psychiatry. 76: 752-5. PMID: 15834044
6. Use of a cell-free system to determine UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities in human hereditary inclusion body myopathy.  |  Sparks, SE., et al. 2005. Glycobiology. 15: 1102-10. PMID: 15987957
7. Late-onset distal myopathy with rimmed vacuoles without mutation in the GNE or dysferlin genes.  |  Suzuki, N., et al. 2005. Muscle Nerve. 32: 812-4. PMID: 16116644
8. The intracellular concentration of sialic acid regulates the polysialylation of the neural cell adhesion molecule.  |  Bork, K., et al. 2005. FEBS Lett. 579: 5079-83. PMID: 16137682

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GLCNE Antibody (H-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-GLCNE Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GLCNE Antibody (H-10) Specifications

Alternate Product Name: GLCNE

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 48-77

Epitope Target: GLCNE

Epitope Species: human

Product Reactivity/Detection: GLCNE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for GLCNE Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin