GLCNE Antibody (G-12): sc-271481

GLCNE Antibody (G-12) is a mouse monoclonal IgM antibody that detects GLCNE in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GLCNE, or UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, plays a crucial role in the biosynthesis of N-acetylneuraminic acid (NeuAc), which is a key precursor for sialic acids. GLCNE is vital for normal sialylation processes in hematopoietic cells, as sialylation is essential for various cellular functions, including cell adhesion, signal transduction, and the metastatic behavior of malignant cells. Notably, GLCNE is upregulated following protein kinase C-dependent phosphorylation and is predominantly expressed in the liver and placenta, with lower levels found in the heart, brain, lung, kidney, skeletal muscle, and pancreas. Deficiencies in GLCNE can lead to serious conditions such as sialuria, inclusion body myopathy 2 (IBM2), and Nonaka myopathy (NM), which are characterized by significant neuromuscular impairments. Sialuria, an autosomal dominant disorder, results from a lack of feedback inhibition of GLCNE by CMP-NeuAc, leading to an overproduction of NeuAc and subsequent accumulation of free sialic acid in the cytoplasm, as well as elevated levels of neuraminic acid in the urine. Both IBM2 and NM are autosomal recessive disorders that manifest as adult-onset muscle weakness, particularly affecting the legs, and are associated with distinctive muscle pathology, including filamentous inclusions and rimmed vacuoles. Anti-GLCNE antibody (G-12) is an invaluable tool for researchers investigating the role of sialylation in health and disease.

The preferred GLCNE antibody is GLCNE Antibody (H-10): sc-376057.