GlcNAc kinase Antibody (G-5): sc-390499
- GlcNAc kinase Antibody (G-5) is a mouse monoclonal IgG1 κ GlcNAc kinase antibody provided at 200 µg/ml
- raised against amino acids 91-390 mapping at the C-terminus of GlcNAc kinase of human origin
- GlcNAc kinase Antibody (G-5) is recommended for detection of GlcNAc kinase of human origin by WB, IP, IF and ELISA
- Anti-GlcNAc kinase Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GlcNAc kinase Antibody (G-5) for WB applications. These reagents are now offered in bundles with GlcNAc kinase Antibody (G-5) (see ordering information below).
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GlcNAc kinase Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects GlcNAc kinase protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GlcNAc kinase antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GlcNAc kinase, also referred to as GNK or N-acetylglucosamine kinase, is a 344 amino acid homodimeric protein that plays a crucial role in amino sugar metabolism, converting N-acetylglucosamine (GlcNAc) into GlcNAc 6-phosphate, which is essential for the synthesis of glycoproteins and glycolipids. This enzymatic activity allows cells to utilize GlcNAc derived from lysosomal degradation or dietary sources, thereby contributing to cellular energy metabolism and maintaining glycan structures. GlcNAc kinase also exhibits ManNAc kinase activity, highlighting a potential role in metabolizing sialic acid precursors. Production of beta anomer phosphorylated sugars by GlcNAc kinase underscores significance in regulating key metabolite availability for various cellular functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GlcNAc kinase Antibody (G-5) References:
- Molecular cloning and characterization of murine and human N-acetylglucosamine kinase. | Hinderlich, S., et al. 2000. Eur J Biochem. 267: 3301-8. PMID: 10824116
- Identification and characterization of the genes for N-acetylglucosamine kinase and N-acetylglucosamine-phosphate deacetylase in the pathogenic fungus Candida albicans. | Yamada-Okabe, T., et al. 2001. Eur J Biochem. 268: 2498-505. PMID: 11298769
- Comparative genomics and experimental characterization of N-acetylglucosamine utilization pathway of Shewanella oneidensis. | Yang, C., et al. 2006. J Biol Chem. 281: 29872-85. PMID: 16857666
- Structures of human N-Acetylglucosamine kinase in two complexes with N-Acetylglucosamine and with ADP/glucose: insights into substrate specificity and regulation. | Weihofen, WA., et al. 2006. J Mol Biol. 364: 388-99. PMID: 17010375
- Mapping active site residues in glutamate-5-kinase. The substrate glutamate and the feed-back inhibitor proline bind at overlapping sites. | Pérez-Arellano, I., et al. 2006. FEBS Lett. 580: 6247-53. PMID: 17069808
- Characterization of ligand binding to N-acetylglucosamine kinase studied by STD NMR. | Blume, A., et al. 2008. Biochemistry. 47: 13138-46. PMID: 19006331
- Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans. | Meglasson, MD., et al. 1983. Proc Natl Acad Sci U S A. 80: 85-9. PMID: 6337376
- Induction of germ tube formation by N-acetyl-D-glucosamine in Candida albicans: uptake of inducer and germinative response. | Mattia, E., et al. 1982. J Bacteriol. 152: 555-62. PMID: 6752114
- Purification and characterization of N-acetylglucosamine kinase from rat liver--comparison with UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase. | Hinderlich, S., et al. 1998. Eur J Biochem. 252: 133-9. PMID: 9523722
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GlcNAc kinase Antibody (G-5) | sc-390499 | 200 µg/ml | $316.00 | |||
GlcNAc kinase Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-527635 | 200 µg Ab; 10 µg BP | $354.00 | |||
GlcNAc kinase Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-533008 | 200 µg Ab; 20 µg BP | $354.00 | |||
GlcNAc kinase Antibody (G-5) AC | sc-390499 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GlcNAc kinase Antibody (G-5) HRP | sc-390499 HRP | 200 µg/ml | $316.00 | |||
GlcNAc kinase Antibody (G-5) FITC | sc-390499 FITC | 200 µg/ml | $330.00 | |||
GlcNAc kinase Antibody (G-5) PE | sc-390499 PE | 200 µg/ml | $343.00 | |||
GlcNAc kinase Antibody (G-5) Alexa Fluor® 488 | sc-390499 AF488 | 200 µg/ml | $357.00 | |||
GlcNAc kinase Antibody (G-5) Alexa Fluor® 546 | sc-390499 AF546 | 200 µg/ml | $357.00 | |||
GlcNAc kinase Antibody (G-5) Alexa Fluor® 594 | sc-390499 AF594 | 200 µg/ml | $357.00 | |||
GlcNAc kinase Antibody (G-5) Alexa Fluor® 647 | sc-390499 AF647 | 200 µg/ml | $357.00 | |||
GlcNAc kinase Antibody (G-5) Alexa Fluor® 680 | sc-390499 AF680 | 200 µg/ml | $357.00 | |||
GlcNAc kinase Antibody (G-5) Alexa Fluor® 790 | sc-390499 AF790 | 200 µg/ml | $357.00 |