Gl Syn Antibody (E-4): sc-74430
- Gl Syn Antibody (E-4) is a mouse monoclonal IgG2a κ Gl Syn antibody, cited in 43 publications, provided at 200 µg/ml
- raised against amino acids 1-373 representing full length Gl Syn of human origin
- Gl Syn Antibody (E-4) is recommended for detection of GI Syn of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Gl Syn Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Gl Syn Antibody (E-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with Gl Syn Antibody (E-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Gl Syn Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Gl Syn protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Gl Syn (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glutamine synthetase, the target of anti-Gl Syn antibody (E-4), plays a crucial role in the brain by catalyzing the conversion of glutamic acid to glutamine, a process vital for neurotransmitter recycling and nitrogen metabolism. Glutamine synthetase is predominantly found in astrocytes, which are essential for maintaining the balance of neurotransmitters in the central nervous system. Glutamine synthetase in astrocytes not only aids in detoxifying excess glutamate, thereby protecting neurons from excitotoxicity, but also supports the metabolic needs of neurons by providing glutamine, which is a precursor for neurotransmitter synthesis. Glutamine synthetase is also expressed in the liver, where Gl Syn (E-4) monoclonal antibody has shown its contribution to nitrogen homeostasis, and overexpression has been implicated in primary liver cancers, suggesting a potential role in hepatocyte transformation. Gl Syn (E-4) monoclonal antibody′s versatility in various applications makes this reagent an invaluable tool for researchers studying glutamine synthetase functions in both neurological and hepatic contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gl Syn Antibody (E-4) References:
- Cloning and functional characterization of the rat glutamine synthetase gene. | Mill, JF., et al. 1991. Brain Res Mol Brain Res. 9: 197-207. PMID: 1674354
- cDNA sequence of the long mRNA for human glutamine synthase. | Van den Hoff, MJ., et al. 1991. Biochim Biophys Acta. 1090: 249-51. PMID: 1681907
- Gliogenesis in the embryonic avian optic tectum: neuronal-glial interactions influence astroglial phenotype maturation. | Linser, PJ. and Perkins, M. 1987. Brain Res. 428: 277-90. PMID: 2881613
- Sequence of a human glutamine synthetase cDNA. | Gibbs, CS., et al. 1987. Nucleic Acids Res. 15: 6293. PMID: 2888076
- Cell contacts are required for induction by cortisol of glutamine synthetase gene transcription in the retina. | Vardimon, L., et al. 1988. Proc Natl Acad Sci U S A. 85: 5981-5. PMID: 2901094
- Overexpression of glutamine synthetase in human primary liver cancer. | Christa, L., et al. 1994. Gastroenterology. 106: 1312-20. PMID: 7909780
- Glutamine synthetase protects against neuronal degeneration in injured retinal tissue. | Gorovits, R., et al. 1997. Proc Natl Acad Sci U S A. 94: 7024-9. PMID: 9192685
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gl Syn Antibody (E-4) | sc-74430 | 200 µg/ml | $322.00 | |||
Gl Syn Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-521110 | 200 µg Ab, 40 µg BP | $361.00 | |||
Gl Syn Antibody (E-4) AC | sc-74430 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Gl Syn Antibody (E-4) HRP | sc-74430 HRP | 200 µg/ml | $322.00 | |||
Gl Syn Antibody (E-4) FITC | sc-74430 FITC | 200 µg/ml | $336.00 | |||
Gl Syn Antibody (E-4) PE | sc-74430 PE | 200 µg/ml | $349.00 | |||
Gl Syn Antibody (E-4) Alexa Fluor® 488 | sc-74430 AF488 | 200 µg/ml | $364.00 | |||
Gl Syn Antibody (E-4) Alexa Fluor® 546 | sc-74430 AF546 | 200 µg/ml | $364.00 | |||
Gl Syn Antibody (E-4) Alexa Fluor® 594 | sc-74430 AF594 | 200 µg/ml | $364.00 | |||
Gl Syn Antibody (E-4) Alexa Fluor® 647 | sc-74430 AF647 | 200 µg/ml | $364.00 | |||
Gl Syn Antibody (E-4) Alexa Fluor® 680 | sc-74430 AF680 | 200 µg/ml | $364.00 | |||
Gl Syn Antibody (E-4) Alexa Fluor® 790 | sc-74430 AF790 | 200 µg/ml | $364.00 |