girdin CRISPR/Cas9 KO Plasmid (m): sc-430846
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- girdin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the girdin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: girdin Antibody (H-6): sc-393757
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
girdin CRISPR/Cas9 KO Plasmid (m) | sc-430846 | 20 µg | $397.00 |
Overview
Mouse Ccdc88a encodes girdin (also known as GIV), a large cytoplasmic scaffold protein that integrates signals from heterotrimeric G proteins and receptor tyrosine kinases to coordinate actin remodeling, cell polarity, and directional migration. Girdin localizes to the leading edge and associates with cytoskeletal regulators to influence processes such as lamellipodia formation, vesicle trafficking, and epithelial organization. It has been linked to PI3K–AKT signaling dynamics and the control of cell growth and survival programs, making it relevant for studies of tissue morphogenesis and pathological cell motility. Dysregulated girdin-associated pathways have been reported in diverse disease contexts where altered adhesion and migration contribute to progression, supporting mechanistic investigation in mouse model systems.
girdin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ccdc88a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ccdc88a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ccdc88a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish girdin protein expression.
This CRISPR knockout system enables efficient generation of Ccdc88a-deficient cell models for investigation of girdin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ccdc88a exon(s) critical for girdin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ccdc88a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by girdin CRISPR/Cas9 KO Plasmid (m) and girdin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ccdc88a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by girdin HDR Plasmid (m) and girdin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ccdc88a homology arms to support homology-directed repair at defined Ccdc88a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.