GILZ Antibody (G-5): sc-133215

GILZ Antibody (G-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects GILZ protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GILZ (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The glucocorticoid-induced leucine zipper (GILZ) protein plays a crucial role in mediating the immunosuppressive effects of glucocorticoid hormones, particularly in T cells, where GILZ expression is induced by dexamethasone. GILZ is primarily expressed in normal lymphocytes found in the thymus, spleen, and lymph nodes, while GILZ is absent in non-lymphoid tissues such as the brain, liver, and kidney. This specific localization is significant as GILZ′s role in immune regulation highlights potential therapeutic targeting in conditions characterized by excessive immune responses. GILZ protects T cells from apoptosis induced by anti-CD3 antibodies by inhibiting the expression of Fas and Fas ligand, and GILZ also interferes with various transcription factors, including Egr-2, Egr-3, and NFAT/AP-1. Notably, GILZ′s interaction with c-Fos and c-Jun inhibits the binding of active AP-1 to its DNA consensus site, while GILZ binding to NFkB subunits prevents their nuclear translocation, further underscoring GILZ′s importance in modulating immune responses. The gene encoding human GILZ is located on chromosome Xq22.3, making GILZ a critical component in understanding immune function and potential therapeutic interventions.

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GILZ Antibody (G-5) References:

1. Cloning, chromosomal assignment and tissue distribution of human GILZ, a glucocorticoid hormone-induced gene.  |  Cannarile, L., et al. 2001. Cell Death Differ. 8: 201-3. PMID: 11313722
2. Inhibition of AP-1 by the glucocorticoid-inducible protein GILZ.  |  Mittelstadt, PR. and Ashwell, JD. 2001. J Biol Chem. 276: 29603-10. PMID: 11397794
3. Modulation of T-cell activation by the glucocorticoid-induced leucine zipper factor via inhibition of nuclear factor kappaB.  |  Ayroldi, E., et al. 2001. Blood. 98: 743-53. PMID: 11468175
4. Glucocorticoid-induced leucine zipper inhibits the Raf-extracellular signal-regulated kinase pathway by binding to Raf-1.  |  Ayroldi, E., et al. 2002. Mol Cell Biol. 22: 7929-41. PMID: 12391160
5. Synthesis of glucocorticoid-induced leucine zipper (GILZ) by macrophages: an anti-inflammatory and immunosuppressive mechanism shared by glucocorticoids and IL-10.  |  Berrebi, D., et al. 2003. Blood. 101: 729-38. PMID: 12393603
6. GILZ as a Mediator of the Anti-Inflammatory Effects of Glucocorticoids.  |  Ronchetti, S., et al. 2015. Front Endocrinol (Lausanne). 6: 170. PMID: 26617572
7. Implicating the Role of GILZ in Glucocorticoid Modulation of T-Cell Activation.  |  Cannarile, L., et al. 2019. Front Immunol. 10: 1823. PMID: 31440237
8. Glucocorticoids and Glucocorticoid-Induced-Leucine-Zipper (GILZ) in Psoriasis.  |  Sevilla, LM. and Pérez, P. 2019. Front Immunol. 10: 2220. PMID: 31572404
9. Glucocorticoid-Induced Leucine Zipper (GILZ) in Cardiovascular Health and Disease.  |  Cappetta, D., et al. 2021. Cells. 10: PMID: 34440924
10. GILZ as a Regulator of Cell Fate and Inflammation.  |  Bruscoli, S., et al. 2021. Cells. 11: PMID: 35011684
11. The role of GILZ in lipid metabolism and adipocyte biology.  |  Chen, S., et al. 2022. Prostaglandins Other Lipid Mediat. 163: 106668. PMID: 35934213
12. A new dexamethasone-induced gene of the leucine zipper family protects T lymphocytes from TCR/CD3-activated cell death.  |  D'Adamio, F., et al. 1997. Immunity. 7: 803-12. PMID: 9430225

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GILZ Antibody (G-5) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-GILZ Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GILZ Antibody (G-5) Specifications

Alternate Product Name: TSC22D3

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-134

Epitope Target: GILZ

Epitope Species: human

Product Reactivity/Detection: GILZ and all other TSC22 family members

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for GILZ Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin