GH Antibody (E-7): sc-374266
- GH Antibody (E-7) is a mouse monoclonal IgG1 κ GH antibody, cited in 10 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 151-189 near the C-terminus of GH of human origin
- GH Antibody (E-7) is recommended for detection of GH-1, GH-2 and Lactogen (chorionic somatommamotropin) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-GH Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GH Antibody (E-7) for WB and IHC(P) applications. These reagents are now offered in bundles with GH Antibody (E-7) (see ordering information below).
QUICK LINKS
GH Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Growth Hormone (GH) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GH antibody (E-7) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. Growth Hormone, also known as somatotropin, is essential for regulating growth, metabolism, and differentiation across various mammalian cell types, primarily by modulating the synthesis of multiple mRNA species. GH (E-7) monoclonal antibody recognizes GH when bound to growth hormone receptor (GHR) on target cells, initiating a phosphorylation cascade that activates numerous signaling pathways critical for cellular function and development. GH is secreted in a pulsatile manner, tightly regulated by the hypothalamic hormones GH-releasing hormone (GHRH) and somatostatin (SRIF), which together control GH synthesis and release from somatotrophs in the anterior pituitary gland. Additionally, GH secretion is subject to feedback regulation by its own levels and by insulin-like growth factor I, ensuring a balanced physiological response. The human growth hormone protein consists of 191 amino acids and features two disulfide bridges, which are crucial for its structural integrity and biological activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GH Antibody (E-7) References:
- Control of growth hormone (GH) release by GH secretagogues. | Robinson, IC. 2000. Novartis Found Symp. 227: 206-20; discussion 220-4. PMID: 10752072
- Evidence for a selective loss of somatostatin receptor subtype expression in male germ cell tumors of seminoma type. | Baou, N., et al. 2000. Carcinogenesis. 21: 805-10. PMID: 10753219
- Growth hormone and colorectal carcinoma: localization of receptors. | Lincoln, DT., et al. 2000. In Vivo. 14: 41-9. PMID: 10757060
- Evolution of the growth hormone-releasing factor (GRF) family of peptides. | Campbell, RM. and Scanes, CG. 1992. Growth Regul. 2: 175-91. PMID: 1290954
- Normal and recombinant human growth hormone administered by constant infusion feminize catechol estrogen formation by rat liver microsomes. | Jellinck, PH., et al. 1985. Endocrinology. 117: 2274-8. PMID: 2998732
- Sequences of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication. | Niall, HD., et al. 1971. Proc Natl Acad Sci U S A. 68: 866-70. PMID: 5279528
- Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24. | Harper, ME., et al. 1982. Am J Hum Genet. 34: 227-34. PMID: 7072716
- The human growth hormone (GH) receptor and its truncated isoform: sulfhydryl group inactivation in the study of receptor internalization and GH-binding protein generation. | Amit, T., et al. 1999. Endocrinology. 140: 266-72. PMID: 9886834
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GH Antibody (E-7) | sc-374266 | 200 µg/ml | $322.00 | |||
GH Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-529704 | 200 µg Ab; 10 µg BP | $361.00 | |||
GH Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-522679 | 200 µg Ab, 40 µg BP | $361.00 | |||
GH Antibody (E-7) AC | sc-374266 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GH Antibody (E-7) HRP | sc-374266 HRP | 200 µg/ml | $322.00 | |||
GH Antibody (E-7) FITC | sc-374266 FITC | 200 µg/ml | $336.00 | |||
GH Antibody (E-7) PE | sc-374266 PE | 200 µg/ml | $349.00 | |||
GH Antibody (E-7) Alexa Fluor® 488 | sc-374266 AF488 | 200 µg/ml | $364.00 | |||
GH Antibody (E-7) Alexa Fluor® 546 | sc-374266 AF546 | 200 µg/ml | $364.00 | |||
GH Antibody (E-7) Alexa Fluor® 594 | sc-374266 AF594 | 200 µg/ml | $364.00 | |||
GH Antibody (E-7) Alexa Fluor® 647 | sc-374266 AF647 | 200 µg/ml | $364.00 | |||
GH Antibody (E-7) Alexa Fluor® 680 | sc-374266 AF680 | 200 µg/ml | $364.00 | |||
GH Antibody (E-7) Alexa Fluor® 790 | sc-374266 AF790 | 200 µg/ml | $364.00 | |||
GH (E-7) Neutralizing Peptide | sc-374266 P | 100 µg/0.5 ml | $69.00 |