GGTase-IIβ Antibody (B-8): sc-365926

GGTase-IIβ Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects GGTase-II beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GGTase-IIβ (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GGTase-II beta protein plays a crucial role in post-translational modification of Rab GTPases, which are essential for intracellular vesicle trafficking and membrane dynamics. Specifically, GGTase-II catalyzes the transfer of two 20-carbon geranylgeranyl groups from geranylgeranyl pyrophosphate to C-terminal cysteine residues of Rab proteins, a modification vital for proper localization and function within the cell. This geranylgeranylation is important for activation of Rab proteins and interaction with effector proteins that mediate various cellular processes, including endocytosis and exocytosis. Proper functioning of Rab GTPases is critical for maintaining cellular homeostasis and facilitating communication between different cellular compartments, making GGTase-II a significant player in cellular signaling pathways.

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GGTase-IIβ Antibody (B-8) References:

1. Stereochemical analysis of the reaction catalyzed by human protein geranylgeranyl transferase.  |  Clausen, VA., et al. 2001. Biochemistry. 40: 3920-30. PMID: 11300771
2. Expression of mammalian geranylgeranyltransferase type-II in Escherichia coli and its application for in vitro prenylation of Rab proteins.  |  Kalinin, A., et al. 2001. Protein Expr Purif. 22: 84-91. PMID: 11388804
3. Phosphoisoprenoids modulate association of Rab geranylgeranyltransferase with REP-1.  |  Thomä, NH., et al. 2001. J Biol Chem. 276: 48637-43. PMID: 11675392
4. Protein prenylation: genes, enzymes, targets, and functions.  |  Schafer, WR. and Rine, J. 1992. Annu Rev Genet. 26: 209-37. PMID: 1482112
5. Thematic review series: lipid posttranslational modifications. Structural biology of protein farnesyltransferase and geranylgeranyltransferase type I.  |  Lane, KT. and Beese, LS. 2006. J Lipid Res. 47: 681-99. PMID: 16477080
6. Genes associated with the tumour microenvironment are differentially expressed in cured versus primary chemotherapy-refractory diffuse large B-cell lymphoma.  |  Linderoth, J., et al. 2008. Br J Haematol. 141: 423-32. PMID: 18419622
7. Protective effects of intrathecal injection of AAV9-RabGGTB-GFP+ in SOD1G93A mice.  |  Gao, T., et al. 2023. Front Aging Neurosci. 15: 1092607. PMID: 36967828
8. RABGGTB plays a critical role in ALS pathogenesis.  |  Ma, H., et al. 2024. Brain Res Bull. 206: 110833. PMID: 38042502
9. Studies of cloning, chromosomal mapping, and embryonic expression of the mouse Rab geranylgeranyl transferase beta subunit.  |  Wei, LN., et al. 1995. Cell Growth Differ. 6: 607-14. PMID: 7544156
10. cDNA cloning and chromosomal localization of the genes encoding the alpha- and beta-subunits of human Rab geranylgeranyl transferase: the 3' end of the alpha-subunit gene overlaps with the transglutaminase 1 gene promoter.  |  van Bokhoven, H., et al. 1996. Genomics. 38: 133-40. PMID: 8954794
11. Single prenyl-binding site on protein prenyl transferases.  |  Desnoyers, L. and Seabra, MC. 1998. Proc Natl Acad Sci U S A. 95: 12266-70. PMID: 9770475
12. Requirement for geranylgeranyl transferase I and acyl transferase in the TGF-beta-stimulated pathway leading to elastin mRNA stabilization.  |  Kucich, U., et al. 1998. Biochem Biophys Res Commun. 252: 111-6. PMID: 9813154

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GGTase-IIβ Antibody (B-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-GGTase-IIβ Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GGTase-IIβ Antibody (B-8) Specifications

Alternate Product Name: GGTase-IIβ

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 139-331

Epitope Target: GGTase-IIβ

Epitope Species: human

Product Reactivity/Detection: GGTase-IIβ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for GGTase-IIβ Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin