Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GGTase-IIβ CRISPR/Cas9 KO Plasmid (h): sc-406913
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GGTase-IIβ CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GGTase-IIβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GGTase-IIβ Antibody (B-8): sc-365926
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GGTase-IIβ CRISPR/Cas9 KO Plasmid (h) | sc-406913 | 20 µg | $397.00 |
Overview
RABGGTB encodes the beta subunit of Rab geranylgeranyltransferase (GGTase-IIβ), a core component of the heterodimeric enzyme that catalyzes geranylgeranylation of Rab GTPases. This lipid modification is essential for Rab membrane association and trafficking functions that govern vesicle budding, endosomal sorting, autophagy-related membrane dynamics, and regulated secretion. Through its impact on Rab-dependent transport routes, GGTase-IIβ influences organelle identity and signaling compartmentalization across the endomembrane system. Dysregulation of Rab prenylation and vesicle trafficking has been linked to neurodegeneration, immune dysfunction, and cancer-associated alterations in membrane transport and receptor recycling.
GGTase-IIβ CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RABGGTB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RABGGTB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RABGGTB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GGTase-IIβ protein expression.
This CRISPR knockout system enables efficient generation of RABGGTB-deficient cell models for investigation of GGTase-IIβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RABGGTB exon(s) critical for GGTase-IIβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RABGGTB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GGTase-IIβ CRISPR/Cas9 KO Plasmid (h) and GGTase-IIβ CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RABGGTB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GGTase-IIβ HDR Plasmid (h) and GGTase-IIβ HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RABGGTB homology arms to support homology-directed repair at defined RABGGTB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.