GGTase-Iα Antibody (B-9): sc-393545
- GGTase-Iα Antibody (B-9) is a mouse monoclonal IgG2a κ GGTase-Iα antibody provided at 200 µg/ml
- raised against amino acids 1-275 mapping at the N-terminus of GGTase-Iα of human origin
- GGTase-I alpha Antibody (B-9) is recommended for detection of GGTase-Iα of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GGTase-I alpha Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GGTase-Iα Antibody (B-9) for WB applications. These reagents are now offered in bundles with GGTase-Iα Antibody (B-9) (see ordering information below).
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GGTase-Iα Antibody (B-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects GGTase-I alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GGTase-Iα antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GGTase-I alpha protein plays a crucial role in post-translational modification of proteins through geranylgeranylation, which is essential for proper functioning of several signaling pathways, including TGF-β1 signaling pathway. This modification occurs at C-terminal CAAX motif of target proteins, where "A" represents an aliphatic amino acid and "X" is typically leucine. Geranylgeranylation process is vital for membrane localization and activity of various proteins, particularly Rab GTPases, which are involved in intracellular trafficking. GGTase-I enzyme is a heterodimer composed of an α subunit and a β subunit, and GGTase-I alpha (B-9) antibody serves as an invaluable tool for researchers studying these pathways and their implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GGTase-Iα Antibody (B-9) References:
- Stereochemical analysis of the reaction catalyzed by human protein geranylgeranyl transferase. | Clausen, VA., et al. 2001. Biochemistry. 40: 3920-30. PMID: 11300771
- Expression of mammalian geranylgeranyltransferase type-II in Escherichia coli and its application for in vitro prenylation of Rab proteins. | Kalinin, A., et al. 2001. Protein Expr Purif. 22: 84-91. PMID: 11388804
- Phosphoisoprenoids modulate association of Rab geranylgeranyltransferase with REP-1. | Thomä, NH., et al. 2001. J Biol Chem. 276: 48637-43. PMID: 11675392
- Protein prenylation: genes, enzymes, targets, and functions. | Schafer, WR. and Rine, J. 1992. Annu Rev Genet. 26: 209-37. PMID: 1482112
- Thematic review series: lipid posttranslational modifications. Structural biology of protein farnesyltransferase and geranylgeranyltransferase type I. | Lane, KT. and Beese, LS. 2006. J Lipid Res. 47: 681-99. PMID: 16477080
- cDNA cloning and chromosomal localization of the genes encoding the alpha- and beta-subunits of human Rab geranylgeranyl transferase: the 3' end of the alpha-subunit gene overlaps with the transglutaminase 1 gene promoter. | van Bokhoven, H., et al. 1996. Genomics. 38: 133-40. PMID: 8954794
- Single prenyl-binding site on protein prenyl transferases. | Desnoyers, L. and Seabra, MC. 1998. Proc Natl Acad Sci U S A. 95: 12266-70. PMID: 9770475
- Requirement for geranylgeranyl transferase I and acyl transferase in the TGF-beta-stimulated pathway leading to elastin mRNA stabilization. | Kucich, U., et al. 1998. Biochem Biophys Res Commun. 252: 111-6. PMID: 9813154
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GGTase-Iα Antibody (B-9) | sc-393545 | 200 µg/ml | $322.00 | |||
GGTase-Iα Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-530489 | 200 µg Ab; 10 µg BP | $361.00 | |||
GGTase-Iα Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-523988 | 200 µg Ab, 40 µg BP | $361.00 | |||
GGTase-Iα Antibody (B-9): m-IgG2a BP-HRP Bundle | sc-547507 | 200 µg Ab; 10 µg BP | $361.00 | |||
GGTase-Iα Antibody (B-9) AC | sc-393545 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GGTase-Iα Antibody (B-9) HRP | sc-393545 HRP | 200 µg/ml | $322.00 | |||
GGTase-Iα Antibody (B-9) FITC | sc-393545 FITC | 200 µg/ml | $336.00 | |||
GGTase-Iα Antibody (B-9) PE | sc-393545 PE | 200 µg/ml | $349.00 | |||
GGTase-Iα Antibody (B-9) Alexa Fluor® 488 | sc-393545 AF488 | 200 µg/ml | $364.00 | |||
GGTase-Iα Antibody (B-9) Alexa Fluor® 546 | sc-393545 AF546 | 200 µg/ml | $364.00 | |||
GGTase-Iα Antibody (B-9) Alexa Fluor® 594 | sc-393545 AF594 | 200 µg/ml | $364.00 | |||
GGTase-Iα Antibody (B-9) Alexa Fluor® 647 | sc-393545 AF647 | 200 µg/ml | $364.00 | |||
GGTase-Iα Antibody (B-9) Alexa Fluor® 680 | sc-393545 AF680 | 200 µg/ml | $364.00 | |||
GGTase-Iα Antibody (B-9) Alexa Fluor® 790 | sc-393545 AF790 | 200 µg/ml | $364.00 |