GGT1 Antibody (E-5): sc-166908

GGT1 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects human GGT1 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GGT1 Antibody (E-5) is available in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. GGT1, or gamma-glutamyltranspeptidase, plays a crucial role in glutathione metabolism by acting as a glutathionase that transfers glutamyl moiety from glutathione to various amino acids and dipeptide acceptors. This enzymatic activity maintains cellular redox balance and detoxifies harmful compounds, making GGT1 essential for cellular defense mechanisms. GGT1 primarily resides on the outer cell membrane surface and appears across mammalian tissues, especially in the liver, where activity increases during certain liver diseases. GGT1 enters the bloodstream following liver damage, and elevated levels serve as an early biomarker for hepatocellular carcinoma. While GGT5 converts leukotriene C4 to leukotriene D4, GGT5 doesn′t act on synthetic substrates used in GGT assays, highlighting GGT1′s specific role. In human serum and tissues, GGT1 shows significant heterogeneity due to different glycosylation patterns rather than gene product variations, emphasizing GGT1′s complex role in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GGT1 Antibody (E-5) References:

1. Identification of a human gamma-glutamyl cleaving enzyme related to, but distinct from, gamma-glutamyl transpeptidase.  |  Heisterkamp, N., et al. 1991. Proc Natl Acad Sci U S A. 88: 6303-7. PMID: 1676842
2. High-level expression of enzymatically active mature human gamma-glutamyltransferase in transgenic V79 Chinese hamster cells.  |  Visvikis, A., et al. 1991. Proc Natl Acad Sci U S A. 88: 7361-5. PMID: 1678521
3. Gene expression levels in normal human lymphoblasts with variable sensitivities to arsenite: identification of GGT1 and NFKBIE expression levels as possible biomarkers of susceptibility.  |  Komissarova, EV., et al. 2008. Toxicol Appl Pharmacol. 226: 199-205. PMID: 17976673
4. Pooling-based genome-wide association study implicates gamma-glutamyltransferase 1 (GGT1) gene in pancreatic carcinogenesis.  |  Diergaarde, B., et al. 2010. Pancreatology. 10: 194-200. PMID: 20484958
5. Biochemical and quantitative proteomics investigations in Arabidopsis ggt1 mutant leaves reveal a role for the gamma-glutamyl cycle in plant's adaptation to environment.  |  Tolin, S., et al. 2013. Proteomics. 13: 2031-45. PMID: 23661340
6. The 5'UTR Intron of Arabidopsis GGT1 Aminotransferase Enhances Promoter Activity by Recruiting RNA Polymerase II.  |  Laxa, M., et al. 2016. Plant Physiol. 172: 313-27. PMID: 27418588
7. Assignment of the human gamma-glutamyl transferase gene to the long arm of chromosome 22.  |  Bulle, F., et al. 1987. Hum Genet. 76: 283-6. PMID: 2885259
8. γ-glutamyl transpeptidase deficiency caused by a large homozygous intragenic deletion in GGT1.  |  Darin, N., et al. 2018. Eur J Hum Genet. 26: 808-817. PMID: 29483667
9. Increased risk of acute pancreatitis occurrence in smokers with rs5751901 polymorphisms in GGT1 gene.  |  Ściskalska, M., et al. 2020. Int J Med Sci. 17: 242-254. PMID: 32038108
10. Hypericum perforatum L. Regulates Glutathione Redox Stress and Normalizes Ggt1/Anpep Signaling to Alleviate OVX-Induced Kidney Dysfunction.  |  Liu, YR., et al. 2021. Front Pharmacol. 12: 628651. PMID: 33981220
11. Targeting GGT1 Eliminates the Tumor-Promoting Effect and Enhanced Immunosuppressive Function of Myeloid-Derived Suppressor Cells Caused by G-CSF.  |  Xie, Z., et al. 2022. Front Pharmacol. 13: 873792. PMID: 35548341
12. Redox Mechanisms in Cisplatin Resistance of Cancer Cells: The Twofold Role of Gamma-Glutamyltransferase 1 (GGT1).  |  Pompella, A., et al. 2022. Front Oncol. 12: 920316. PMID: 35669424

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GGT1 Antibody (E-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-GGT1 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GGT1 Antibody (E-5) Specifications

Alternate Product Name: GGT1

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 156-360

Epitope Target: GGT1

Epitope Species: human

Product Reactivity/Detection: GGT1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for GGT1 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin