Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GGPS1 CRISPR/Cas9 KO Plasmid (h): sc-404003
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GGPS1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GGPS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GGPS1 Antibody (E-1): sc-271680
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GGPS1 CRISPR/Cas9 KO Plasmid (h) | sc-404003 | 20 µg | $397.00 |
Overview
GGPS1 encodes geranylgeranyl diphosphate synthase 1, a key enzyme in the mevalonate pathway that produces geranylgeranyl diphosphate (GGPP), an essential isoprenoid lipid precursor. GGPP supports protein prenylation, enabling membrane association and signaling activity of small GTPases such as RAS, RHO, and RAB family members, thereby influencing vesicle trafficking, cytoskeletal dynamics, and proliferation. By regulating isoprenoid flux and prenylation capacity, GGPS1 contributes to metabolic control of mitochondrial and ER-associated processes and intersects with cholesterol and dolichol biosynthesis. Dysregulation of the mevalonate–prenylation axis and altered GGPS1 activity have been linked to oncogenic signaling dependencies and other disorders involving lipid metabolism and protein trafficking.
GGPS1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GGPS1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GGPS1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GGPS1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GGPS1 protein expression.
This CRISPR knockout system enables efficient generation of GGPS1-deficient cell models for investigation of GGPS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GGPS1 exon(s) critical for GGPS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GGPS1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GGPS1 CRISPR/Cas9 KO Plasmid (h) and GGPS1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GGPS1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GGPS1 HDR Plasmid (h) and GGPS1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GGPS1 homology arms to support homology-directed repair at defined GGPS1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.