GGA3 Antibody (8): sc-135923

GGA3 Antibody (8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the GGA3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-GGA3 antibody (8) is available as the non-conjugated format. GGA3 (8) antibody recognizes a member of the Golgi-localized, γ-Adaptin ear-containing, ARF-binding protein family, which plays a crucial role in intracellular protein trafficking, specifically facilitating the transport of soluble proteins from the trans-Golgi network to endosomes and lysosomes. This process is vital for maintaining cellular homeostasis and ensuring that proteins reach their correct destinations, which is essential for various cellular functions, including signaling and metabolism. GGA3 interacts with sorting receptors and clathrin, utilizing its multi-domain structure that includes an N-terminal VHS domain, a GAT domain, a hinge segment, and a C-terminal GAE domain. The VHS domain is particularly important as it recognizes specific sequences on sorting receptors, allowing for the precise sorting of proteins. The human GGA3 gene is located on chromosome 17 and encodes a 723 amino acid protein that shares significant sequence identity with its family members, GGA1 and GGA2, highlighting its evolutionary conservation and functional importance in cellular processes.

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GGA3 Antibody (8) References:

1. A family of proteins with gamma-adaptin and VHS domains that facilitate trafficking between the trans-Golgi network and the vacuole/lysosome.  |  Hirst, J., et al. 2000. J Cell Biol. 149: 67-80. PMID: 10747088
2. GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex.  |  Dell'Angelica, EC., et al. 2000. J Cell Biol. 149: 81-94. PMID: 10747089
3. Structural basis for recognition of acidic-cluster dileucine sequence by GGA1.  |  Shiba, T., et al. 2002. Nature. 415: 937-41. PMID: 11859376
4. GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors.  |  Takatsu, H., et al. 2002. Biochem J. 365: 369-78. PMID: 11950392
5. Autoinhibition of the ligand-binding site of GGA1/3 VHS domains by an internal acidic cluster-dileucine motif.  |  Doray, B., et al. 2002. Proc Natl Acad Sci U S A. 99: 8072-7. PMID: 12060753
6. Cooperation of GGAs and AP-1 in packaging MPRs at the trans-Golgi network.  |  Doray, B., et al. 2002. Science. 297: 1700-3. PMID: 12215646

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GGA3 Antibody (8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GGA3 Antibody (8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GGA3 Antibody (8) Specifications

Alternate Product Name: GGA3

Product Clone Name: 8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 424-542

Epitope Target: GGA3

Epitope Species: human

Product Reactivity/Detection: GGA3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GGA3 Antibody (8): N/A