GFP Antibody (1A5): sc-101536

GFP Antibody (1A5) is a rat monoclonal IgG1 antibody specifically raised against recombinant green fluorescent protein (GFP), a 27 kDa protein originating from the bioluminescent jellyfish Aequorea victoria. GFP′s fluorescence is a direct result of its unique autocatalytic post-translational modification, where the tripeptide sequence Ser65-Tyr66-Gly67 undergoes cyclization, dehydration, and oxidation to form a covalently bonded chromophore. This modification, occurring spontaneously without the need for external enzymes or cofactors, is central to GFP′s intrinsic fluorescence. The chromophore formation involves a sequence of complex chemical transformations, including nucleophilic attack and oxidation, leading to the creation of a conjugated system responsible for light absorption and emission. Additional factors such as the protein′s tertiary structure and proper folding are critical for facilitating this self-catalyzed modification, as they position the tripeptide residues in the correct orientation for cyclization. Mutations at or near the chromophore-forming sequence can alter the efficiency or spectral properties of the modification, which has been exploited to develop GFP variants with tailored fluorescence characteristics. Variants like EGFP have been engineered for enhanced brightness, photostability, and pH resistance, making them invaluable for high-resolution imaging in fluorescence microscopy and quantitative assays in flow cytometry (FCM). GFP′s role as a reporter protein extends across applications involving gene expression analysis, protein tracking, and cellular imaging. Anti-GFP antibody (1A5) is widely used for detecting GFP-tagged proteins in techniques such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), supporting a broad range of research in molecular and cellular biology.

The preferred GFP antibody is GFP Antibody (B-2): sc-9996.