Date published: 2026-3-17

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GFP Antibody (15): sc-101525

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Datasheets
  • GFP Antibody (15) is a mouse monoclonal IgG1 κ, cited in 24 publications, provided at 200 µg/ml
  • raised against recombinant GFP
  • recommended for detection of GFP by IF and IHC(P)
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GFP Antibody (15). This work is in progress.
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GFP Antibody (15) is a mouse monoclonal IgG1 kappa light chain antibody developed against recombinant Green Fluorescent Protein (GFP), also referred to as Aequorea victoria GFP or Emerald GFP in certain variants. GFP is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria, renowned for its ability to emit bright green fluorescence when exposed to blue to ultraviolet light. This fluorescence is a result of GFP′s distinctive β-barrel structure, which consists of 11 β-strands that form a stable barrel enclosing an internal α-helix housing the chromophore. The β-barrel not only stabilizes the chromophore but also protects it from quenching interactions, ensuring efficient and reliable fluorescence. Anti-GFP antibody (15) is optimized for use in immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP), enabling precise detection and localization of GFP-tagged proteins in various biological samples. GFP monoclonal antibody (15) facilitates the study of protein expression, localization, and interactions in live cells and tissues, making it an essential tool for researchers in molecular and cellular biology. Anti-GFP antibody (15) supports accurate monitoring of gene expression and protein dynamics in diverse experimental contexts by targeting GFP, which is widely employed as a fusion tag due to its non-invasive fluorescent properties.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

GFP Antibody (15)

sc-101525
200 µg/ml
$322.00

Is this antibody also recognizing YFP?

Asked by: Adrian31
Thank you for your question,. The antibody does not detect YFP.
Answered by: BlakeJ
Date published: 2023-08-09

You recommended the GFP Antibody (15): sc-101525 for detection of GFP by IHC(P) but I see no picture and cannot find any published data? Could you share your data or help me find published papers. I am OK to test a free sample of the antibody. Thx

Asked by: UCRes
Thank you for your question. We recommend this mouse monoclonal antibody, GFP (15): sc-101525, for detection of GFP by IF and IHC(P) based on publications in the literature. For one reference, please refer to the publication below on PubMed, PMID: 20099318, which is also listed as a product citation on our website (with a link out to PubMed). We are not currently offering a free sample size for this particular antibody. As an alternative, if you would like to test a free sample of GFP Antibody (B-2): sc-9996, please contact our Technical Service Department. This antibody is not currently recommended for IHC(P) because we have not yet tested the antibody with FFPE, however, it also has been published for this application and may be suitable.
Answered by: Technical Support
Date published: 2017-09-11

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with GFP Antibody (15): sc-101525?

Asked by: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 3
Date published: 2017-02-03
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Rated 5 out of 5 by from Great antibodyWorked very well with Western blotting and IP. Would highly recommend this antibody.
Date published: 2019-05-10
Rated 4 out of 5 by from GOODI had this one to test the CONSTRUCT, and it worked well in HEK lysate
Date published: 2018-05-14
Rated 5 out of 5 by from Really good antibodyGot this as sample.Now ordering it for future use.
Date published: 2017-12-16
Rated 5 out of 5 by from Good effectGFP used for detect tumor cell, IF of this antibody is obvious.
Date published: 2017-09-06
Rated 5 out of 5 by from Good Antibody!We used this antibody for IF staining and WB, and it has got great results with a nice clear image.
Date published: 2017-09-04
Rated 3 out of 5 by from One work, one didn'tfirst one I bought didn't work. Then the tech person sent me two more, same one I picked still not work. the other one he suggested worked.
Date published: 2017-03-23
Rated 5 out of 5 by from Great IF stainWe used this antibody for IF staining and got great results with a nice clear image
Date published: 2017-01-10
Rated 5 out of 5 by from This antibody is good for Western BlotI used GFP-tag antibody at a dilution of 1:2000 in PBST for 1 h and is enough for an excellent Western blot signal when the protein from DF-1 cell lysate.
Date published: 2016-11-24
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GFP Antibody (15) is rated 4.8 out of 5 by 12.
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