Gemin4 Antibody (E-8): sc-365424
- Gemin4 Antibody (E-8) is a mouse monoclonal IgG1 κ Gemin4 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1019-1053 at the C-terminus of Gemin4 of human origin
- Gemin4 Antibody (E-8) is recommended for detection of Gemin4 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Gemin4 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Gemin4 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Gemin4 Antibody (E-8) (see ordering information below).
Gemin4 (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Gemin4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). Anti-Gemin4 antibody (E-8) is available as non-conjugated form, as well as multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Gemin4 is a component of the SMN core complex which, while in the cytoplasm, plays an essential role in ribonucleoprotein (snRNP) assembly, including biogenesis, delivery and recycling of snRNPs to the spliceosome. In the nucleus, where SMN is required for pre-mRNA splicing, Gemin4 concentrates next to coiled bodies in subnuclear structures called gems, which are highly enriched in splicosomal snRNPs, and in the nucleolus. Deletion or loss-of-function mutations in SMN lead to the neurodegenerative disease spinal muscular atrophy (SMA). Human Gemin4 maps to chromosome 17p13.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gemin4 Antibody (E-8) References:
- Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli. | Charroux, B., et al. 2000. J Cell Biol. 148: 1177-86. PMID: 10725331
- Association of galectin-1 and galectin-3 with Gemin4 in complexes containing the SMN protein. | Park, JW., et al. 2001. Nucleic Acids Res. 29: 3595-602. PMID: 11522829
- SMN interacts with a novel family of hnRNP and spliceosomal proteins. | Mourelatos, Z., et al. 2001. EMBO J. 20: 5443-52. PMID: 11574476
- HCC-associated protein HCAP1, a variant of GEMIN4, interacts with zinc-finger proteins. | Di, Y., et al. 2003. J Biochem. 133: 713-8. PMID: 12869526
- Nucleocytoplasmic lectins. | Wang, JL., et al. 2004. Biochim Biophys Acta. 1673: 75-93. PMID: 15238251
- Understanding the biochemical activities of galectin-1 and galectin-3 in the nucleus. | Patterson, RJ., et al. 2002. Glycoconj J. 19: 499-506. PMID: 14758073
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gemin4 Antibody (E-8) | sc-365424 | 200 µg/ml | $322.00 | |||
Gemin4 Antibody (E-8): m-IgG Fc BP-HRP Bundle | sc-529373 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gemin4 Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-522242 | 200 µg Ab, 40 µg BP | $361.00 | |||
Gemin4 Antibody (E-8): m-IgG1 BP-HRP Bundle | sc-543485 | 200 µg Ab; 20 µg BP | $361.00 | |||
Gemin4 Antibody (E-8) AC | sc-365424 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Gemin4 Antibody (E-8) HRP | sc-365424 HRP | 200 µg/ml | $322.00 | |||
Gemin4 Antibody (E-8) FITC | sc-365424 FITC | 200 µg/ml | $336.00 | |||
Gemin4 Antibody (E-8) PE | sc-365424 PE | 200 µg/ml | $349.00 | |||
Gemin4 Antibody (E-8) Alexa Fluor® 488 | sc-365424 AF488 | 200 µg/ml | $364.00 | |||
Gemin4 Antibody (E-8) Alexa Fluor® 546 | sc-365424 AF546 | 200 µg/ml | $364.00 | |||
Gemin4 Antibody (E-8) Alexa Fluor® 594 | sc-365424 AF594 | 200 µg/ml | $364.00 | |||
Gemin4 Antibody (E-8) Alexa Fluor® 647 | sc-365424 AF647 | 200 µg/ml | $364.00 | |||
Gemin4 Antibody (E-8) Alexa Fluor® 680 | sc-365424 AF680 | 200 µg/ml | $364.00 | |||
Gemin4 Antibody (E-8) Alexa Fluor® 790 | sc-365424 AF790 | 200 µg/ml | $364.00 | |||
Gemin4 (E-8) Neutralizing Peptide | sc-365424 P | 100 µg/0.5 ml | $69.00 |