GDNF Antibody (B-8): sc-13147

GDNF Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects glial cell line-derived neurotrophic factor (GDNF) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GDNF (B-8) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. GDNF plays a crucial role in the survival and differentiation of midbrain dopaminergic neurons, which are essential for motor control and are significantly affected in conditions like Parkinson′s disease. GDNF is primarily secreted and acts on neurons through GFRα1 receptor, which is located on the surface of target cells. This interaction is vital for neuronal health, as GDNF activates signaling pathways that promote cell survival and growth. GDNF′s location in the extracellular space allows effective binding to receptors and exerts neuroprotective effects, making GDNF a key player in neurodevelopment and potential therapeutic target for neurodegenerative diseases.

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GDNF Antibody (B-8) References:

1. Isolation of male germ-line stem cells; influence of GDNF.  |  Hofmann, MC., et al. 2005. Dev Biol. 279: 114-24. PMID: 15708562
2. Direct interactions among Ret, GDNF and GFRalpha1 molecules reveal new insights into the assembly of a functional three-protein complex.  |  Amoresano, A., et al. 2005. Cell Signal. 17: 717-27. PMID: 15722196
3. Effect of GDNF on differentiation of cultured ventral mesencephalic dopaminergic and non-dopaminergic calretinin-expressing neurons.  |  Schaller, B., et al. 2005. Brain Res. 1036: 163-72. PMID: 15725414
4. GDNF and GFRalpha1 promote differentiation and tangential migration of cortical GABAergic neurons.  |  Pozas, E. and Ibáñez, CF. 2005. Neuron. 45: 701-13. PMID: 15748846
5. GDNF and protection of adult central catecholaminergic neurons.  |  Pascual, A., et al. 2011. J Mol Endocrinol. 46: R83-92. PMID: 21357726
6. Transforming growth factor-beta. A family of growth regulatory peptides.  |  Miller, DA., et al. 1990. Ann N Y Acad Sci. 593: 208-17. PMID: 2375595
7. Identification of another member of the transforming growth factor type beta gene family.  |  ten Dijke, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 4715-9. PMID: 3164476
8. GDNF synthesis, signaling, and retrograde transport in motor neurons.  |  Cintrón-Colón, AF., et al. 2020. Cell Tissue Res. 382: 47-56. PMID: 32897420
9. GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment.  |  Ma, J., et al. 2023. Adv Healthc Mater. 12: e2202377. PMID: 36549669
10. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells.  |  Derynck, R., et al. Nature. 316: 701-5. PMID: 3861940
11. Clonal cell lines from the rat central nervous system.  |  Schubert, D., et al. 1974. Nature. 249: 224-7. PMID: 4151463
12. GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.  |  Lin, LF., et al. 1993. Science. 260: 1130-2. PMID: 8493557

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GDNF Antibody (B-8) has 52 citations in a variety of scientific publications.

Primary antibodies like Anti-GDNF Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GDNF Antibody (B-8) Specifications

Alternate Product Name: Glial cell derived neurotrophic factor (GDNF)

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 78-134

Epitope Target: GDNF

Epitope Species: human

Product Reactivity/Detection: GDNF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 52 citations in various scientific publications

Available Conjugates/Forms for GDNF Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin