GCP2 Antibody (F-3): sc-377117
- GCP2 Antibody (F-3) is a mouse monoclonal IgG2b κ GCP2 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 383-423 within an internal region of GCP2 of human origin
- GCP2 Antibody (F-3) is recommended for detection of γ-tubulin complex component 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GCP2 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GCP2 Antibody (F-3) for WB applications. These reagents are now offered in bundles with GCP2 Antibody (F-3) (see ordering information below).
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GCP2 Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects GCP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCP2 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GCP2, also known as Gamma-tubulin complex component 2, plays a crucial role in microtubule organization by localizing to the centrosome, where GCP2 is essential for microtubule nucleation. This process is vital for proper cell division and maintaining the structural integrity of the cytoskeleton, which in turn affects cellular shape, motility, and intracellular transport. GCP2 is part of the γ-Tubulin complex, which includes other associated proteins such as GCP3, GCP4, GCP5, and GCP6, each contributing distinct functions that ensure the complex operates effectively. The proper functioning of the γ-Tubulin complex is critical for the formation of microtubule organizing centers, and any disruption in this process can lead to severe cellular dysfunction and has been implicated in various diseases, including cancer. GCP2, a 902 amino acid protein, is ubiquitously expressed and can exist as soluble complexes in the cytoplasm, ready to be recruited to the centrosome as needed, highlighting GCP2′s dynamic role in cellular organization and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GCP2 Antibody (F-3) References:
- Human 76p: A new member of the gamma-tubulin-associated protein family. | Fava, F., et al. 1999. J Cell Biol. 147: 857-68. PMID: 10562286
- An unappreciated role for RNA surveillance. | Hillman, RT., et al. 2004. Genome Biol. 5: R8. PMID: 14759258
- Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. | Rush, J., et al. 2005. Nat Biotechnol. 23: 94-101. PMID: 15592455
- Microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. | Delgehyr, N., et al. 2005. J Cell Sci. 118: 1565-75. PMID: 15784680
- GCP-WD is a gamma-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule nucleation. | Lüders, J., et al. 2006. Nat Cell Biol. 8: 137-47. PMID: 16378099
- Donor centrosome regulation of initial spindle formation in mouse somatic cell nuclear transfer: roles of gamma-tubulin and nuclear mitotic apparatus protein 1. | Van Thuan, N., et al. 2006. Biol Reprod. 74: 777-87. PMID: 16407502
- PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates. | Stirling, PC., et al. 2006. J Biol Chem. 281: 7012-21. PMID: 16415341
- Fluorescence resonance energy transfer and molecular modeling studies on 4',6-diamidino-2-phenylindole (DAPI) complexes with tubulin. | Arbildua, JJ., et al. 2006. Protein Sci. 15: 410-9. PMID: 16452620
- The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p. | Murphy, SM., et al. 1998. J Cell Biol. 141: 663-74. PMID: 9566967
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCP2 Antibody (F-3) | sc-377117 | 200 µg/ml | $322.00 | |||
GCP2 Antibody (F-3): m-IgGκ BP-HRP Bundle | sc-523194 | 200 µg Ab, 40 µg BP | $361.00 | |||
GCP2 Antibody (F-3): m-IgG2b BP-HRP Bundle | sc-549095 | 200 µg Ab; 10 µg BP | $361.00 | |||
GCP2 Antibody (F-3) AC | sc-377117 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GCP2 Antibody (F-3) HRP | sc-377117 HRP | 200 µg/ml | $322.00 | |||
GCP2 Antibody (F-3) FITC | sc-377117 FITC | 200 µg/ml | $336.00 | |||
GCP2 Antibody (F-3) PE | sc-377117 PE | 200 µg/ml | $349.00 | |||
GCP2 Antibody (F-3) Alexa Fluor® 488 | sc-377117 AF488 | 200 µg/ml | $364.00 | |||
GCP2 Antibody (F-3) Alexa Fluor® 546 | sc-377117 AF546 | 200 µg/ml | $364.00 | |||
GCP2 Antibody (F-3) Alexa Fluor® 594 | sc-377117 AF594 | 200 µg/ml | $364.00 | |||
GCP2 Antibody (F-3) Alexa Fluor® 647 | sc-377117 AF647 | 200 µg/ml | $364.00 | |||
GCP2 Antibody (F-3) Alexa Fluor® 680 | sc-377117 AF680 | 200 µg/ml | $364.00 | |||
GCP2 Antibody (F-3) Alexa Fluor® 790 | sc-377117 AF790 | 200 µg/ml | $364.00 | |||
GCP2 (F-3) Neutralizing Peptide | sc-377117 P | 100 µg/0.5 ml | $69.00 |