Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GCNF CRISPR/Cas9 KO Plasmid (h): sc-406967
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GCNF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GCNF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GCNF Antibody (B-2): sc-271733
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCNF CRISPR/Cas9 KO Plasmid (h) | sc-406967 | 20 µg | $397.00 |
Overview
NR6A1 encodes germ cell nuclear factor (GCNF), an orphan nuclear receptor that functions primarily as a transcriptional repressor during embryonic development and germ cell biology. GCNF contributes to lineage specification and maintenance of differentiation programs by binding response elements in target promoters and recruiting corepressor complexes that alter chromatin state. In human cells, NR6A1-regulated networks intersect with pluripotency and differentiation circuitry, including repression of genes such as POU5F1/OCT4, linking it to epigenetic control of developmental transitions. Dysregulated NR6A1 activity has been investigated in contexts involving aberrant differentiation and transcriptional reprogramming, making it relevant for studies of developmental disorders and tumor cell plasticity.
GCNF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NR6A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NR6A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NR6A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GCNF protein expression.
This CRISPR knockout system enables efficient generation of NR6A1-deficient cell models for investigation of GCNF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NR6A1 exon(s) critical for GCNF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NR6A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GCNF CRISPR/Cas9 KO Plasmid (h) and GCNF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NR6A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GCNF HDR Plasmid (h) and GCNF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NR6A1 homology arms to support homology-directed repair at defined NR6A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.