GCNF Antibody (B-2) recommended for detection of GCNF of mouse, rat and human origin by WB, IP, IF and ELISA

GCNF Antibody (B-2): sc-271733

GCNF Antibody (B-2) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-GCNF Antibody (B-2) is a mouse monoclonal IgG2a (kappa light chain) GCNF antibody provided at 200 µg/ml
    • raised against amino acids 181-480 mapping at the C-terminus of GCNF of human origin
    • Anti-GCNF Antibody (B-2) is recommended for detection of GCNF of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-GCNF Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271733 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GCNF (B-2): sc-271733.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with GCNF (B-2): sc-271733 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced nice Western blot data of GCNF expression Produced nice Western blot data of GCNF expression in HEK293, K-562 and F9 whole cell lysates. -SCBT QC
Date published: 2014-08-29
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