GCN5 Antibody (A-11): sc-365321

GCN5 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect GCN5, also known as KAT2A, across multiple species including human, mouse, rat, canine, and porcine. Anti-GCN5 antibody (A-11) targets a precise epitope within amino acids 363-387 at the N-terminus of the human GCN5 protein, ensuring robust performance in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GCN5 plays a critical role as a histone acetyltransferase, facilitating the acetylation of lysine residues on histone tails, which leads to chromatin remodeling and the regulation of gene transcription. This function is essential for various cellular processes including cell cycle progression, differentiation, and response to environmental signals. By acetylating histones, GCN5 enhances the accessibility of transcription factors to DNA, promoting active gene expression, whereas counterpart histone deacetylases like HDAC1 and HDAC2 are involved in transcriptional repression. GCN5 monoclonal antibody (A-11) recognizes GCN5 across different species, making anti-GCN5 antibody (A-11) a versatile tool for researchers studying epigenetic regulation and transcriptional control mechanisms. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, GCN5 (A-11) antibody provides flexibility for diverse experimental setups.

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GCN5 Antibody (A-11) References:

1. Repression of GCN5 expression or activity attenuates c-MYC expression in non-small cell lung cancer.  |  Mustachio, LM., et al. 2019. Am J Cancer Res. 9: 1830-1845. PMID: 31497362
2. Autophagic degradation of KAT2A/GCN5 promotes directional migration of vascular smooth muscle cells by reducing TUBA/α-tubulin acetylation.  |  Ouyang, C., et al. 2020. Autophagy. 16: 1753-1770. PMID: 31878840
3. The Ada2/Ada3/Gcn5/Sgf29 histone acetyltransferase module.  |  Espinola-Lopez, JM. and Tan, S. 2021. Biochim Biophys Acta Gene Regul Mech. 1864: 194629. PMID: 32890768
4. Alignment of human KAT2A (GCN5) histone acetyltransferase and SARS-CoV-2 Orf8 viral proteins.  |  Lehrer, S. and Rheinstein, PH. 2023. Chronic Dis Transl Med. 9: 263-265. PMID: 36721422
5. Nucleosome structural changes due to acetylation.  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
6. A positive role for histone acetylation in transcription factor access to nucleosomal DNA.  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
7. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
8. Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation.  |  Brownell, JE., et al. 1996. Cell. 84: 843-51. PMID: 8601308
9. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p.  |  Taunton, J., et al. 1996. Science. 272: 408-11. PMID: 8602529
10. A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A.  |  Yang, XJ., et al. 1996. Nature. 382: 319-24. PMID: 8684459
11. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3.  |  Yang, WM., et al. 1996. Proc Natl Acad Sci U S A. 93: 12845-50. PMID: 8917507
12. Identification of mouse histone deacetylase 1 as a growth factor-inducible gene.  |  Bartl, S., et al. 1997. Mol Cell Biol. 17: 5033-43. PMID: 9271381

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GCN5 Antibody (A-11) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-GCN5 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GCN5 Antibody (A-11) Specifications

Alternate Product Name: GCN5

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 363-387

Epitope Target: GCN5

Epitope Species: human

Product Reactivity/Detection: GCN5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for GCN5 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent