GCAP1 Antibody (E-5): sc-390695

GCAP1 Antibody (E-5) is a mouse monoclonal IgG1 antibody that detects guanylate cyclase-activating protein 1 (GCAP1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCAP1 plays a crucial role in the recovery of the dark state of rod photoreceptors after exposure to light by mediating the intracellular stimulation of guanylate cyclase, which is essential for the synthesis of cyclic guanosine monophosphate (c-GMP) in photoreceptors. GCAP1 is localized in the outer segments of rod and cone photoreceptors, where phototransduction occurs, highlighting its importance in visual signaling. Notably, GCAP1 is unique among calcium-binding proteins in the calmodulin superfamily because its calcium-free form actively stimulates the effector enzyme, a feature that underscores its regulatory role in phototransduction. Molecular cloning studies have revealed that mammalian GCAPs, including GCAP1, exhibit over 90% similarity, consisting of 201 to 205 amino acids and containing three conserved calcium-binding sites, which are critical for their function. Additionally, GCAP2, a related protein resulting from a gene duplication event, is exclusively found in the retina, further emphasizing the specialized functions of GCAP proteins in visual processes.

The preferred GCAP1 antibody is GCAP1 Antibody (C-1): sc-390679.