GCAP1 Antibody (C-1): sc-390679
- GCAP1 Antibody (C-1) is a mouse monoclonal IgG2b κ GCAP1 antibody provided at 200 µg/ml
- raised against amino acids 1-201 representing full length GCAP1 of human origin
- GCAP1 Antibody (C-1) is recommended for detection of GCAP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GCAP1 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GCAP1 Antibody (C-1) for WB applications. These reagents are now offered in bundles with GCAP1 Antibody (C-1) (see ordering information below).
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GCAP1 Antibody (C-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects GCAP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCAP1 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The guanylate cyclase-activating protein 1 (GCAP1) plays a crucial role in the phototransduction pathway, particularly in the recovery of rod photoreceptors after exposure to light, by mediating the intracellular stimulation of guanylate cyclase through calcium binding. GCAP1 is primarily located in the outer segments of rod and cone photoreceptors, where GCAP1 is essential for converting light signals into electrical signals, thus enabling vision. Notably, GCAP1 is unique among calcium-binding proteins in the calmodulin superfamily because GCAP1′s calcium-free form actively stimulates guanylate cyclase, leading to the synthesis of cyclic guanosine monophosphate (c-GMP), a vital second messenger in phototransduction. The molecular structure of GCAP1 consists of 201 to 205 amino acids and features three conserved calcium-binding sites, which are critical for function. Additionally, GCAP2, a related protein that arises from a gene duplication event, is exclusively found in the retina, highlighting the specialized role of GCAP proteins in visual processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GCAP1 Antibody (C-1) References:
- Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration. | Sokal, I., et al. 1999. J Biol Chem. 274: 19829-37. PMID: 10391927
- Guanylyl cyclase activating protein. A calcium-sensitive regulator of phototransduction. | Gorczyca, WA., et al. 1995. J Biol Chem. 270: 22029-36. PMID: 7665624
- Molecular characterization of human and mouse photoreceptor guanylate cyclase-activating protein (GCAP) and chromosomal localization of the human gene. | Subbaraya, I., et al. 1994. J Biol Chem. 269: 31080-9. PMID: 7983048
- Localization of guanylate cyclase-activating protein 2 in mammalian retinas. | Otto-Bruc, A., et al. 1997. Proc Natl Acad Sci U S A. 94: 4727-32. PMID: 9114059
- The human GCAP1 and GCAP2 genes are arranged in a tail-to-tail array on the short arm of chromosome 6 (p21.1). | Surguchov, A., et al. 1997. Genomics. 39: 312-22. PMID: 9119368
- Changes in biological activity and folding of guanylate cyclase-activating protein 1 as a function of calcium. | Rudnicka-Nawrot, M., et al. 1998. Biochemistry. 37: 248-57. PMID: 9425045
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCAP1 Antibody (C-1) | sc-390679 | 200 µg/ml | $322.00 | |||
GCAP1 Antibody (C-1): m-IgG Fc BP-HRP Bundle | sc-530223 | 200 µg Ab; 10 µg BP | $361.00 | |||
GCAP1 Antibody (C-1): m-IgGκ BP-HRP Bundle | sc-523633 | 200 µg Ab, 40 µg BP | $361.00 | |||
GCAP1 Antibody (C-1) AC | sc-390679 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GCAP1 Antibody (C-1) HRP | sc-390679 HRP | 200 µg/ml | $322.00 | |||
GCAP1 Antibody (C-1) FITC | sc-390679 FITC | 200 µg/ml | $336.00 | |||
GCAP1 Antibody (C-1) PE | sc-390679 PE | 200 µg/ml | $349.00 | |||
GCAP1 Antibody (C-1) Alexa Fluor® 488 | sc-390679 AF488 | 200 µg/ml | $364.00 | |||
GCAP1 Antibody (C-1) Alexa Fluor® 546 | sc-390679 AF546 | 200 µg/ml | $364.00 | |||
GCAP1 Antibody (C-1) Alexa Fluor® 594 | sc-390679 AF594 | 200 µg/ml | $364.00 | |||
GCAP1 Antibody (C-1) Alexa Fluor® 647 | sc-390679 AF647 | 200 µg/ml | $364.00 | |||
GCAP1 Antibody (C-1) Alexa Fluor® 680 | sc-390679 AF680 | 200 µg/ml | $364.00 | |||
GCAP1 Antibody (C-1) Alexa Fluor® 790 | sc-390679 AF790 | 200 µg/ml | $364.00 |