GC-C Antibody (537) recommended for detection of GC-C of human origin by WB, IP and ELISA

GC-C Antibody (537): sc-100302

GC-C Antibody (537) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-GC-C Antibody (537) is a mouse monoclonal IgG2b (kappa light chain) GC-C antibody provided at 100 µg/ml
    • raised against partial recombinant protein mapping at the N-terminus of GC-C of human origin
    • recommended for detection of GC-C of human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GC-C (537): sc-100302.

How much protein do you recommend loading on the gel for Western blot detection using GC-C (537): sc-100302?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 3
Date published: 2017-02-08
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Rated 5 out of 5 by from Good for WB in mice We used GC-C (537) antibody for WB in Mouse lung primary fibroblast, and it worked very well. We used anti-mouse IgG.
Date published: 2018-03-28
Rated 5 out of 5 by from Good for WB! This monoclonal GC-C Antibody (537) is a good choice for Western blotting applications in human samples.
Date published: 2017-01-27
Rated 5 out of 5 by from T84 colon cancer cell line T84 colon cancer cell line, the staining detected with Flow Citometry was fine
Date published: 2015-09-07
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