GBF1 Antibody (25): sc-136240

GBF1 Antibody (25) is a mouse monoclonal IgG1 kappa light chain antibody that detects GBF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-GBF1 antibody (25) is available as a non-conjugated format, providing researchers with a reliable tool for studying this critical protein. GBF1 protein plays a vital role in maintaining Golgi morphology and facilitating protein trafficking to the membrane, which is essential for various cellular processes, including secretion and membrane dynamics. GBF1 functions as a guanine nucleotide exchange factor (GEF) that activates ADP-ribosylation factors, thereby promoting the formation of COPI-coated vesicles from the cis-Golgi. This function is crucial, especially in the presence of brefeldin A (BFA), a compound that typically disrupts Golgi function by inhibiting GEFs. GBF1 enables cells to sustain normal Golgi structure and function even in the presence of BFA, highlighting its importance in cellular resilience and adaptability. The gene encoding human GBF1 is located on chromosome 10q24, featuring a centrally positioned sec7 domain and a proline-rich C-terminus, which has been shown to interact functionally with p115 protein, further underscoring the intricate regulatory mechanisms involved in Golgi dynamics.

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GBF1 Antibody (25) References:

1. GBF1: A novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5.  |  Claude, A., et al. 1999. J Cell Biol. 146: 71-84. PMID: 10402461
2. GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat.  |  Kawamoto, K., et al. 2002. Traffic. 3: 483-95. PMID: 12047556
3. The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor.  |  García-Mata, R. and Sztul, E. 2003. EMBO Rep. 4: 320-5. PMID: 12634853
4. ADP-ribosylation factor/COPI-dependent events at the endoplasmic reticulum-Golgi interface are regulated by the guanine nucleotide exchange factor GBF1.  |  García-Mata, R., et al. 2003. Mol Biol Cell. 14: 2250-61. PMID: 12808027
5. Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi.  |  Niu, TK., et al. 2005. Mol Biol Cell. 16: 1213-22. PMID: 15616190
6. Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24.  |  Mansour, SJ., et al. 1998. Genomics. 54: 323-7. PMID: 9828135

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GBF1 Antibody (25) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-GBF1 Antibody (25) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GBF1 Antibody (25) Specifications

Alternate Product Name: GBF1

Product Clone Name: 25

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1266-1379

Epitope Target: GBF1

Epitope Species: human

Product Reactivity/Detection: GBF1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for GBF1 Antibody (25): N/A