G_β Antibody (A-4): sc-166250

Gβ Antibody (A-4) is a mouse monoclonal IgG1 antibody that detects Gβ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gβ is a crucial component of heterotrimeric G proteins, which play a vital role in transmitting signals from cell surface receptors to intracellular effectors, thereby influencing various physiological processes. The Gβ subunits, particularly Gβ5, exhibit unique structural characteristics that distinguish them from other β subunits, with Gβ5 showing only 50% homology to Gβ1-4. This structural divergence is significant as Gβ5 interacts with specific signaling pathways and regulatory proteins, thereby modulating the activity of G protein alpha subunits and influencing downstream signaling cascades. Gβ5 is predominantly expressed in the brain, pancreas, kidney, and heart, highlighting Gβ5′s importance in both central and peripheral signaling mechanisms. anti-Gβ antibody (A-4) serves as an essential tool for researchers exploring the intricate roles of Gβ in cellular signaling and biological processes.

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G_β Antibody (A-4) References:

1. Identification of residues in the N terminus of alpha1B critical for inhibition of the voltage-dependent calcium channel by Gbeta gamma.  |  Cantí, C., et al. 1999. J Neurosci. 19: 6855-64. PMID: 10436043
2. Histamine H(1)-receptor activation of nuclear factor-kappa B: roles for G beta gamma- and G alpha(q/11)-subunits in constitutive and agonist-mediated signaling.  |  Bakker, RA., et al. 2001. Mol Pharmacol. 60: 1133-42. PMID: 11641442
3. Graded contribution of the Gbeta gamma binding domains to GIRK channel activation.  |  Sadja, R., et al. 2002. Proc Natl Acad Sci U S A. 99: 10783-8. PMID: 12124401
4. The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits.  |  Harashima, T. and Heitman, J. 2002. Mol Cell. 10: 163-73. PMID: 12150916
5. Roles of G beta gamma in membrane recruitment and activation of p110 gamma/p101 phosphoinositide 3-kinase gamma.  |  Brock, C., et al. 2003. J Cell Biol. 160: 89-99. PMID: 12507995
6. The p101 subunit of PI3Kγ restores activation by Gβ mutants deficient in stimulating p110γ.  |  Shymanets, A., et al. 2012. Biochem J. 441: 851-8. PMID: 22054284
7. Dissecting Arabidopsis Gβ signal transduction on the protein surface.  |  Jiang, K., et al. 2012. Plant Physiol. 159: 975-83. PMID: 22570469
8. Quantitative Multiple-Reaction Monitoring Proteomic Analysis of Gβ and Gγ Subunits in C57Bl6/J Brain Synaptosomes.  |  Yim, YY., et al. 2017. Biochemistry. 56: 5405-5416. PMID: 28880079
9. Localization of G alpha 0 to growth cones in PC12 cells: role of G alpha 0 association with receptors and G beta gamma.  |  Nüsse, O. and Neer, EJ. 1996. J Cell Sci. 109 (Pt 1): 221-8. PMID: 8834806
10. Selectively enhanced cellular signaling by Gi proteins in essential hypertension. G alpha i2, G alpha i3, G beta 1, and G beta 2 are not mutated.  |  Pietruck, F., et al. 1996. Circ Res. 79: 974-83. PMID: 8888689

The preferred G_β antibody is G_β Antibody (H-1): sc-166123.