G_{β 4} Antibody (F-3): sc-374383

Gβ 4 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects G beta 4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-G beta 4 antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G beta 4 protein plays a crucial role in heterotrimeric G protein function, essential for transmitting signals from cell surface receptors to intracellular effectors. G beta 4 protein participates in various physiological processes, including sensory perception, immune responses, and neurotransmission. G beta 4 (F-3) monoclonal antibody targets a subunit known to interact with specific G protein alpha subunits and certain receptors, modulating their activity and influencing downstream signaling pathways. This regulatory function maintains cellular homeostasis and responds to external stimuli. G beta 4 protein′s ability to bind and regulate G protein alpha subunits makes G beta 4 (F-3) antibody an invaluable tool for studying G protein signaling mechanisms in various biological contexts.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

G_{β 4} Antibody (F-3) References:

1. Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes.  |  Hurowitz, EH., et al. 2000. DNA Res. 7: 111-20. PMID: 10819326
2. Different beta-subunits determine G-protein interaction with transmembrane receptors.  |  Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
3. Activation of cytosolic phosphoinositide phospholipase C by G-protein beta gamma subunits.  |  Blank, JL., et al. 1992. J Biol Chem. 267: 23069-75. PMID: 1331076
4. Signaling cross-talk from Gbeta4 subunit to Elk-1 in the rapid action of androgens.  |  Zagar, Y., et al. 2004. J Biol Chem. 279: 2403-13. PMID: 14602719
5. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
6. Region-specific reduction of Gbeta4 expression and induction of the phosphorylation of PKB/Akt and ERK1/2 by aging in rat brain.  |  Song, GY., et al. 2007. Pharmacol Res. 56: 295-302. PMID: 17765569
7. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
8. G protein diversity is increased by associations with a variety of gamma subunits.  |  Gautam, N., et al. 1990. Proc Natl Acad Sci U S A. 87: 7973-7. PMID: 2122451
9. A novel missense variant (Gln220Arg) of GNB4 encoding guanine nucleotide-binding protein, subunit beta-4 in a Japanese family with autosomal dominant motor and sensory neuropathy.  |  Miura, S., et al. 2017. Eur J Med Genet. 60: 474-478. PMID: 28642160
10. Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human.  |  Blatt, C., et al. 1988. Proc Natl Acad Sci U S A. 85: 7642-6. PMID: 2902634
11. A suppressive role of guanine nucleotide-binding protein subunit beta-4 inhibited by DNA methylation in the growth of anti-estrogen resistant breast cancer cells.  |  Wang, B., et al. 2018. BMC Cancer. 18: 817. PMID: 30103729
12. Guanine nucleotide-binding protein subunit beta-4 promotes gastric cancer progression via activating Erk1/2.  |  Gao, J., et al. 2020. Acta Biochim Biophys Sin (Shanghai). 52: 975-987. PMID: 32747927

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{β 4} Antibody (F-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{β 4} Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{β 4} Antibody (F-3) Specifications

Alternate Product Name: G protein subunit beta 4

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 197-225

Epitope Target: G_{β 4}

Epitope Species: human

Product Reactivity/Detection: G_{β 4}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for G_{β 4} Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred G_β antibody is G_β Antibody (H-1): sc-166123.