Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GATA-6 Antibody (3H3-H6-H11): sc-517554. Western blot analysis of GATA-6 expression in HeLa (A), PC-3 (B) and MCF7 (C) nuclear extracts. Detection reagent used: m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GATA-6 CRISPR/Cas9 KO Plasmid (h): sc-400724
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GATA-6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GATA-6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GATA-6 Antibody (3H3-H6-H11): sc-517554
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GATA-6 CRISPR/Cas9 KO Plasmid (h) | sc-400724 | 20 µg | $397.00 |