GART Antibody (G-5): sc-166448

GART Antibody (G-5) is a mouse monoclonal IgG2b antibody that detects GART in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GART, or GAR transformylase, is a pivotal folate-dependent trifunctional enzyme composed of 1,010 amino acids, playing a crucial role in the de novo biosynthesis of purines, which are essential for energy metabolism, cell signaling, and cellular reproduction. This enzyme exhibits three distinct activities: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, and AICAR (phosphoribosylaminoimidazole synthetase), making GART indispensable for the synthesis of nucleotides necessary for DNA and RNA production. The importance of GART is underscored by conservation across vertebrates and expression of two isoforms resulting from alternative splicing, which may have implications in various biological processes and disease states. Given that cancer cells have an increased demand for purines to support rapid proliferation, GART has emerged as a significant target for cancer chemotherapy, highlighting potential as a therapeutic intervention point. Anti-GART antibody (G-5) is an essential tool for researchers investigating the role of purine metabolism in health and disease.

The preferred GART antibody is GART Antibody (D-4): sc-166379.