GART Antibody (D-4): sc-166379

GART Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects GART protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GART (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GART protein, also known as GAR transformylase, is a crucial folate-dependent trifunctional enzyme that plays a significant role in de novo purine biosynthesis, possessing activities as a phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, and AICAR (phosphoribosylaminoimidazole synthetase). GART protein is essential for purine synthesis, which drives energy metabolism, cell signaling, and cellular reproduction, while serving as precursors for coenzymes and regulatory factors involved in RNA and DNA synthesis. Cancer cells exhibit an increased demand for purines to support rapid growth, making GART protein a potential target for cancer chemotherapy. GART protein is highly conserved across vertebrates, and alternative splicing results in the expression of two isoforms, highlighting evolutionary significance and functional versatility in cellular processes.

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GART Antibody (D-4) References:

1. Design, synthesis, and biological evaluation of fluoronitrophenyl substituted folate analogues as potential inhibitors of GAR transformylase and AICAR transformylase.  |  Boger, DL., et al. 2000. Bioorg Med Chem Lett. 10: 1471-5. PMID: 10888335
2. The Unexpected Catalytic Properties of a Heterodimer of GAR Transformylase.  |  Liu, C., et al. 2000. Bioorg Chem. 28: 316-23. PMID: 11352469
3. The apo and ternary complex structures of a chemotherapeutic target: human glycinamide ribonucleotide transformylase.  |  Dahms, TE., et al. 2005. Biochemistry. 44: 9841-50. PMID: 16026156
4. Structural and mechanistic studies on the HeLa and chicken liver proteins that catalyze glycinamide ribonucleotide synthesis and formylation and aminoimidazole ribonucleotide synthesis.  |  Daubner, SC., et al. 1986. Biochemistry. 25: 2951-7. PMID: 3718932
5. Role of folate dependent transformylases in synthesis of purine in bone marrow of man and in bone marrow and liver of rats.  |  Deacon, R., et al. 1985. J Clin Pathol. 38: 1349-52. PMID: 4078017
6. Direct transfer of one-carbon units in the transformylations of de novo purine biosynthesis.  |  Smith, GK., et al. 1982. Biochemistry. 21: 2870-4. PMID: 7104299
7. Assembly of an active enzyme by the linkage of two protein modules.  |  Nixon, AE., et al. 1997. Proc Natl Acad Sci U S A. 94: 1069-73. PMID: 9037007
8. The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome.  |  Brodsky, G., et al. 1997. Hum Mol Genet. 6: 2043-50. PMID: 9328467
9. The human trifunctional enzyme of de novo purine biosynthesis: heterologous expression, purification, and preliminary characterization.  |  Poch, MT., et al. 1998. Protein Expr Purif. 12: 17-24. PMID: 9473452

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GART Antibody (D-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GART Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GART Antibody (D-4) Specifications

Alternate Product Name: GART

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 61-360

Epitope Target: GART

Epitope Species: human

Product Reactivity/Detection: GART

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GART Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin