GAPDH Antibody (D-6): sc-166545

GAPDH Antibody (D-6) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect glyceraldehyde-3-phosphate dehydrogenase (GAPDH) across multiple species, including mouse, rat, human, equine, canine, bovine, and porcine, through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-GAPDH antibody (D-6) targets a peptide located near the C-terminus of human GAPDH. GAPDH is a pivotal enzyme in the glycolytic pathway, catalyzing the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, which is essential for cellular energy production. Beyond its central role in metabolism, GAPDH is involved in a variety of non-glycolytic functions, including membrane fusion, microtubule bundling, DNA repair, and the regulation of apoptosis. These diverse functions make GAPDH a critical player in numerous cellular processes, including cellular metabolism, cancer cell proliferation, and response to hypoxic conditions. GAPDH interacts with Akt, where phosphorylation by Akt enhances glycolytic activity, thereby supporting cancer cell survival under stress. Additionally, GAPDH interacts with hypoxia-inducible factor 1-alpha (HIF-1α), which increases glycolysis in low-oxygen environments, a key mechanism in tumor progression. GAPDH monoclonal antibody (D-6) is available in both non-conjugated form and various conjugated forms, providing versatility for diverse experimental needs and facilitating advanced research into the multifaceted roles of GAPDH in cellular metabolism and disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAPDH Antibody (D-6) has 156 citations in a variety of scientific publications.

Primary antibodies like Anti-GAPDH Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAPDH Antibody (D-6) Specifications

Alternate Product Name: GAPDH

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 265-278

Epitope Target: GAPDH

Epitope Species: human

Product Reactivity/Detection: GAPDH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 156 citations in various scientific publications

Available Conjugates/Forms for GAPDH Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred GAPDH antibody is GAPDH Antibody (6C5): sc-32233.