GAPDH Antibody (6C5): sc-32233

GAPDH Antibody (6C5) is a mouse monoclonal IgG1 kappa light chain antibody raised against GAPDH purified from muscle of rabbit origin, demonstrating reactivity with mouse, rat, human, and rabbit species in applications like western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pivotal enzyme in glycolysis, catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate in the presence of inorganic phosphate and NAD⁺, a critical step in carbohydrate metabolism that yields cellular energy. Beyond its metabolic role, GAPDH is multifunctional, participating in transcriptional activation, RNA binding, nuclear RNA export, DNA replication, and repair, underscoring its importance in various cellular processes. GAPDH predominantly resides in the cytoplasm but can translocate to the nucleus, especially during apoptosis, highlighting its involvement in cell death pathways. Post-translational modifications such as S-nitrosylation, phosphorylation, acetylation, ubiquitination, and ADP-ribosylation modulate GAPDH′s activity and localization, affecting processes like apoptosis and neurodegeneration. Elevated GAPDH expression is observed in liver, lung, and prostate cancers, making GAPDH a valuable biomarker in oncology research. Additionally, GAPDH interacts with neuronal proteins implicated in neurodegenerative disorders, including the β-amyloid precursor protein and huntingtin, emphasizing GAPDH′s significance in neurological studies. GAPDH (6C5) antibody is an indispensable tool for researchers conducting advanced studies in metabolism, cancer biology, and neurodegenerative diseases.

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GAPDH Antibody (6C5) References:

1. Engineered glycolytic glyceraldehyde-3-phosphate dehydrogenase binds the anti conformation of NAD+ nicotinamide but does not experience A-specific hydride transfer.  |  Eyschen, J., et al. 1999. Arch Biochem Biophys. 364: 219-27. PMID: 10190977
2. New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase.  |  Sirover, MA. 1999. Biochim Biophys Acta. 1432: 159-84. PMID: 10407139
3. Glyceraldehyde-3-phosphate dehydrogenase and apoptosis.  |  Berry, MD. and Boulton, AA. 2000. J Neurosci Res. 60: 150-4. PMID: 10740219
4. Glyceraldehyde-3-phosphate dehydrogenase in neurodegeneration and apoptosis signaling.  |  Tatton, WG., et al. 2000. J Neural Transm Suppl. 77-100. PMID: 11205159
5. Transcriptional profiling of cell lines derived from an orthotopic pancreatic tumor model reveals metastasis-associated genes.  |  Tarbé, N., et al. 2001. Anticancer Res. 21: 3221-8. PMID: 11848476
6. Alteration of intracellular structure and function of glyceraldehyde-3-phosphate dehydrogenase: a common phenotype of neurodegenerative disorders?  |  Mazzola, JL. and Sirover, MA. 2002. Neurotoxicology. 23: 603-9. PMID: 12428732
7. Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in late pathological stage human prostate cancer.  |  Rondinelli, RH., et al. 1997. Prostate Cancer Prostatic Dis. 1: 66-72. PMID: 12496918
8. GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues.  |  Barber, RD., et al. 2005. Physiol Genomics. 21: 389-95. PMID: 15769908
9. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase.  |  Meyer-Siegler, K., et al. 1991. Proc Natl Acad Sci U S A. 88: 8460-4. PMID: 1924305
10. GAPDH: a common enzyme with uncommon functions.  |  Nicholls, C., et al. 2012. Clin Exp Pharmacol Physiol. 39: 674-9. PMID: 21895736
11. GAPDH in neuroblastoma: Functions in metabolism and survival.  |  Cornett, K., et al. 2022. Front Oncol. 12: 979683. PMID: 36267982
12. GAPDH: A common housekeeping gene with an oncogenic role in pan-cancer.  |  Wang, J., et al. 2023. Comput Struct Biotechnol J. 21: 4056-4069. PMID: 37664172

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAPDH Antibody (6C5) has 4,773 citations in a variety of scientific publications.

Primary antibodies like Anti-GAPDH Antibody (6C5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAPDH Antibody (6C5) Specifications

Alternate Product Name: GAPDH

Product Clone Name: 6C5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GAPDH

Epitope Species: rabbit

Product Reactivity/Detection: GAPDH

Species Reactivity/Detection: mouse, rat, human, rabbit

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4,773 citations in various scientific publications

Available Conjugates/Forms for GAPDH Antibody (6C5): N/A