GAPDH Antibody (0411): sc-47724

GAPDH Antibody (0411) is a mouse monoclonal IgG1 kappa light chain antibody raised against recombinant glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of human origin. GAPDH is a critical enzyme in glycolysis, catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, an essential step in carbohydrate metabolism that generates energy for the cell. While primarily located in the cytoplasm, GAPDH can translocate to the nucleus during apoptosis, where it participates in nuclear functions such as transcription activation, DNA replication, and DNA repair, underscoring its multifunctional role in cellular processes. GAPDH interacts with several significant proteins, including the β-amyloid precursor protein (APP), which is implicated in the pathogenesis of Alzheimer′s disease; Huntingtin, the mutated protein in Huntington′s disease, indicating a role in neurodegenerative disorders; and Siah1, an E3 ubiquitin ligase involved in apoptosis, highlighting its participation in cell death pathways. Additionally, GAPDH expression is upregulated in liver, lung, and prostate cancers, making these tissues suitable positive controls for GAPDH (0411) monoclonal antibody. anti-GAPDH antibody (0411) is reactive with human samples and is ideal for applications such as western blotting (WB) and immunoprecipitation (IP). With high specificity and affinity, GAPDH monoclonal antibody (0411) is an excellent tool for researchers investigating glycolytic pathways, nuclear functions of GAPDH, its interactions with disease-related proteins, and its role in cancer, thereby significantly advancing research in these critical areas.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAPDH Antibody (0411) has 3,428 citations in a variety of scientific publications.

Primary antibodies like Anti-GAPDH Antibody (0411) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAPDH Antibody (0411) Specifications

Alternate Product Name: GAPDH

Product Clone Name: 0411

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: GAPDH

Epitope Species: human

Product Reactivity/Detection: GAPDH

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3,428 citations in various scientific publications

Available Conjugates/Forms for GAPDH Antibody (0411): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GAPDH antibody is GAPDH Antibody (6C5): sc-32233.