GAP-43 Antibody (7B10): sc-33705

GAP-43 Antibody (7B10) is a mouse monoclonal IgG2a kappa light chain antibody that detects GAP-43 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GAP-43 Antibody (7B10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GAP-43, also known as growth-associated protein 43, plays a vital role in neuronal development and regeneration, being predominantly expressed in growth cones during these processes. GAP-43 presence is crucial for axonal outgrowth and synapse formation, which are essential for proper neuronal communication and repair following injury. GAP-43 localization in the axonal membrane underscores GAP-43′s importance in facilitating dynamic changes required for neuronal plasticity and regeneration. Additionally, GAP-43 interacts with specific proteins, such as the neuron-specific Elav/Hu family member HuD, which stabilizes GAP-43 mRNA, thereby enhancing protein levels during neuronal development. Notably, studies have shown that heterozygous GAP-43 knockout mice, which exhibit a 50% reduction in GAP-43 levels, demonstrate significant impairments in memory and sensory perception, highlighting GAP-43′s critical role in cognitive functions and neural signaling.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GAP-43 Antibody (7B10) References:

1. GAP-43 as a plasticity protein in neuronal form and repair.  |  Strittmatter, SM., et al. 1992. J Neurobiol. 23: 507-20. PMID: 1431834
2. The influence of GAP-43 on orientation of cell division through G proteins.  |  Huang, R., et al. 2015. Int J Dev Neurosci. 47: 333-9. PMID: 26380950
3. Cerebrospinal fluid GAP-43 in early multiple sclerosis.  |  Rot, U., et al. 2018. Mult Scler J Exp Transl Clin. 4: 2055217318792931. PMID: 30094057
4. GAP-43 and BASP1 in Axon Regeneration: Implications for the Treatment of Neurodegenerative Diseases.  |  Chung, D., et al. 2020. Front Cell Dev Biol. 8: 567537. PMID: 33015061
5. GAP-43 ameliorates Podocyte injury by decreasing nuclear NFATc1 expression.  |  Lian, Z., et al. 2021. Biochem Biophys Rep. 28: 101145. PMID: 34746448
6. Prognostic value of cutaneous reinnervation with GAP-43 in oxaliplatin-induced neuropathy.  |  Albayrak, M., et al. 2022. J Neurol. 269: 4174-4184. PMID: 35258850
7. Amphetamine-induced neurite injury in PC12 cells through inhibiting GAP-43 pathway.  |  Yan, X., et al. 2022. Neurotoxicology. 93: 103-111. PMID: 36150536
8. Association of CSF GAP-43 With the Rate of Cognitive Decline and Progression to Dementia in Amyloid-Positive Individuals.  |  Öhrfelt, A., et al. 2023. Neurology. 100: e275-e285. PMID: 36192174
9. Association of CSF GAP-43 and APOE ε4 with Cognition in Mild Cognitive Impairment and Alzheimer's Disease.  |  Zhu, Y., et al. 2022. Cells. 12: PMID: 36611808
10. GAP-43 closely interacts with BDNF in hippocampal neurons and is associated with Alzheimer's disease progression.  |  Lee, YJ., et al. 2023. Front Mol Neurosci. 16: 1150399. PMID: 37143467

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAP-43 Antibody (7B10) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-GAP-43 Antibody (7B10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAP-43 Antibody (7B10) Specifications

Alternate Product Name: GAP-43

Product Clone Name: 7B10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: GAP-43

Epitope Species: rat

Product Reactivity/Detection: axonal membrane protein GAP-43

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for GAP-43 Antibody (7B10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GAP-43 antibody is GAP-43 Antibody (B-5): sc-17790.