γ1-Adaptin Antibody (F-10): sc-398867

γ1-Adaptin Antibody (F-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects gamma 1-Adaptin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-gamma 1-Adaptin antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. gamma 1-Adaptin plays a crucial role in forming clathrin-coated vesicles, which are essential for receptor-mediated endocytosis, a process vital for cellular uptake of nutrients and signaling molecules. gamma 1-Adaptin is primarily located at the trans-Golgi network and plasma membrane, where gamma 1-Adaptin interacts with clathrin and other adaptor proteins to facilitate vesicle transport. Adapter complexes, such as AP-1 and AP-2, are composed of clathrin-binding adaptin proteins, including gamma and beta1 for AP-1, and alpha and beta2 for AP-2, along with smaller subunits like AP50 and AP17. Alpha and beta adaptin chains feature a two-domain architecture with variable C-terminal domains, significant for functional diversity and tissue-specific expression. Splice variants of alpha-adaptin exhibit differential expression levels across various tissues, highlighting the complexity of clathrin-mediated transport mechanisms. Understanding gamma 1-Adaptin dynamics and interactions with other proteins, such as AP180 and CALM, is essential for elucidating molecular underpinnings of endocytosis and implications in cellular physiology.

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γ1-Adaptin Antibody (F-10) References:

1. Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain.  |  Nogi, T., et al. 2002. Nat Struct Biol. 9: 527-31. PMID: 12042876
2. Conservation and diversity in families of coated vesicle adaptins.  |  Ponnambalam, S., et al. 1990. J Biol Chem. 265: 4814-20. PMID: 1969413
3. Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus.  |  Robinson, MS. 1990. J Cell Biol. 111: 2319-26. PMID: 2126014
4. Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2.  |  Kirchhausen, T., et al. 1989. Proc Natl Acad Sci U S A. 86: 2612-6. PMID: 2495531
5. Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins).  |  Robinson, MS. 1989. J Cell Biol. 108: 833-42. PMID: 2564002
6. A hypomorphic mutation of the gamma-1 adaptin gene (Ap1g1) causes inner ear, retina, thyroid, and testes abnormalities in mice.  |  Johnson, KR., et al. 2016. Mamm Genome. 27: 200-12. PMID: 27090238
7. De novo and bi-allelic variants in AP1G1 cause neurodevelopmental disorder with developmental delay, intellectual disability, and epilepsy.  |  Usmani, MA., et al. 2021. Am J Hum Genet. 108: 1330-1341. PMID: 34102099
8. Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs.  |  Rebendenne, A., et al. 2022. Nat Genet. 54: 1090-1102. PMID: 35879413
9. Expression and localization of alpha-adaptin isoforms.  |  Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
10. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site.  |  Morris, SA., et al. 1993. EMBO J. 12: 667-75. PMID: 8440257
11. The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.  |  Dreyling, MH., et al. 1996. Proc Natl Acad Sci U S A. 93: 4804-9. PMID: 8643484
12. Endocytosis and molecular sorting.  |  Mellman, I. 1996. Annu Rev Cell Dev Biol. 12: 575-625. PMID: 8970738

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γ1-Adaptin Antibody (F-10) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-γ1-Adaptin Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γ1-Adaptin Antibody (F-10) Specifications

Alternate Product Name: γ1-Adaptin

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 5-27

Epitope Target: γ1-Adaptin

Epitope Species: mouse

Product Reactivity/Detection: γ1-Adaptin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for γ1-Adaptin Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin