γ-GCSm Antibody (E-4): sc-55586

γ-GCSm Antibody (E-4) is a mouse monoclonal IgG2a antibody that detects γ-GCSm protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). γ-GCSm (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The gamma-glutamylcysteine synthetase (γ-GCS) protein plays a crucial role as the rate-limiting enzyme in glutathione synthesis, a vital antioxidant that protects cells from oxidative stress and maintains cellular redox balance. γ-GCS protein is a heterodimer composed of a heavy catalytic subunit and a light regulatory subunit, which together respond to various physiological stimuli such as inflammation and oxidative stress. The human γ-GCS gene encoding the 367 amino acid catalytic subunit is located on chromosome 6p12, while the gene for the regulatory subunit is found on chromosome 1p22-p21. The formation of a reversible disulfide bond between the two subunits enhances γ-GCS enzyme′s activity, highlighting the importance of post-translational modifications in regulating function. Anti-γ-GCSm antibody (E-4) enables researchers to effectively study the dynamics of glutathione synthesis and its implications in various biological processes and diseases.

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γ-GCSm Antibody (E-4) References:

1. Regulation of gamma-glutamylcysteine synthetase expression in response to oxidative stress.  |  Kondo, T., et al. 1999. Free Radic Res. 31: 325-34. PMID: 10517537
2. Multi-faceted regulation of gamma-glutamylcysteine synthetase.  |  Soltaninassab, SR., et al. 2000. J Cell Physiol. 182: 163-70. PMID: 10623879
3. Regulation of gamma-glutamylcysteine synthetase subunit gene expression: insights into transcriptional control of antioxidant defenses.  |  Wild, AC. and Mulcahy, RT. 2000. Free Radic Res. 32: 281-301. PMID: 10741850
4. Regulation of redox glutathione levels and gene transcription in lung inflammation: therapeutic approaches.  |  Rahman, I. and MacNee, W. 2000. Free Radic Biol Med. 28: 1405-20. PMID: 10924859
5. Inflammation and the regulation of glutathione level in lung epithelial cells.  |  Rahman, I. 1999. Antioxid Redox Signal. 1: 425-47. PMID: 11233143
6. Gamma-glutamylcysteine synthetase-glutathione synthetase: domain structure and identification of residues important in substrate and glutathione binding.  |  Janowiak, BE., et al. 2006. Biochemistry. 45: 10461-73. PMID: 16939198
7. Gamma-glutamylcysteine synthetase mediates the c-Myc-dependent response to antineoplastic agents in melanoma cells.  |  Benassi, B., et al. 2007. Mol Pharmacol. 72: 1015-23. PMID: 17628013
8. γ-Glutamylcysteine synthetase (γ-GCS) as a target for overcoming chemo- and radio-resistance of human hepatocellular carcinoma cells.  |  Lin, LC., et al. 2018. Life Sci. 198: 25-31. PMID: 29549912
9. Gamma-glutamylcysteine synthetase and tryparedoxin 1 exert high control on the antioxidant system in Trypanosoma cruzi contributing to drug resistance and infectivity.  |  González-Chávez, Z., et al. 2019. Redox Biol. 26: 101231. PMID: 31203195
10. γ-Glutamylcysteine synthetase and γ-glutamyl transferase as differential enzymatic sources of γ-glutamylpeptides in mice.  |  Kobayashi, S., et al. 2020. Amino Acids. 52: 555-566. PMID: 32170467
11. Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.  |  Sierra-Rivera, E., et al. 1995. Cytogenet Cell Genet. 70: 278-9. PMID: 7789189
12. Glutathione: an overview of biosynthesis and modulation.  |  Anderson, ME. 1998. Chem Biol Interact. 111-112: 1-14. PMID: 9679538

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γ-GCSm Antibody (E-4) has 44 citations in a variety of scientific publications.

Primary antibodies like Anti-γ-GCSm Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γ-GCSm Antibody (E-4) Specifications

Alternate Product Name: GCLM

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 1-274

Epitope Target: γ-GCSm

Epitope Species: human

Product Reactivity/Detection: γ-GCSm

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 44 citations in various scientific publications

Available Conjugates/Forms for γ-GCSm Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin