γ-GCSc Antibody (D-4): sc-166382

γ-GCSc Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects γ-GCSc (γ-glutamylcysteine synthetase heavy subunit) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). γ-GCSc is a crucial enzyme playing a vital role in glutathione biosynthesis, essential for cellular defense against oxidative stress and maintaining redox homeostasis. γ-GCSc is primarily located in hemocytes, brain, liver, and kidney, where γ-GCSc forms a heterodimer with a regulatory subunit, γ-GCSm (γ-glutamylcysteine synthetase light subunit), to create the γ-GCS complex. γ-GCS complex is the first enzyme in the glutathione biosynthetic pathway and is responsible for the rate-limiting step, making γ-GCS a key player in cellular detoxification processes. γ-GCSc regulation is influenced by various factors, including oxidants and heavy metals, which can upregulate transcription, thereby enhancing glutathione production in response to cellular stress. Notably, mutations such as the His370Leu change in γ-GCSc can lead to significant deficiencies in enzyme activity, resulting in conditions like hemolytic anemia and reduced glutathione levels in red blood cells. γ-GCSc (D-4) antibody is an invaluable tool for researchers studying oxidative stress responses and glutathione metabolism in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

γ-GCSc Antibody (D-4) References:

1. Knockout of the mouse glutamate cysteine ligase catalytic subunit (Gclc) gene: embryonic lethal when homozygous, and proposed model for moderate glutathione deficiency when heterozygous.  |  Dalton, TP., et al. 2000. Biochem Biophys Res Commun. 279: 324-9. PMID: 11118286
2. The role of H2O2 in the platelet-derived growth factor-induced transcription of the gamma-glutamylcysteine synthetase heavy subunit.  |  Catarzi, S., et al. 2002. Cell Mol Life Sci. 59: 1388-94. PMID: 12363041
3. Delayed mechanism for induction of gamma-glutamylcysteine synthetase heavy subunit mRNA stability by oxidative stress involving p38 mitogen-activated protein kinase signaling.  |  Song, IS., et al. 2005. J Biol Chem. 280: 28230-40. PMID: 15946948
4. Cigarette Smoke-Induced Hypermethylation of the GCLC Gene Is Associated With COPD.  |  Cheng, L., et al. 2016. Chest. 149: 474-482. PMID: 26087411
5. Polymorphism of Glutamate-Cysteine Ligase Subunit Catalytic (GCLC) Gene in Pulmonary Tuberculosis Patients.  |  Yuniastuti, A., et al. 2017. Pak J Biol Sci. 20: 397-402. PMID: 29023060
6. Effects of CeO2 nanoparticles on the HO-1, NQO1, and GCLC expression in the testes of diabetic rats.  |  Hasanvand, D., et al. 2018. Can J Physiol Pharmacol. 96: 963-969. PMID: 29894645
7. Association of Polymorphisms of Glutamate Cysteine Ligase Genes GCLC C-129 T and GCLM C-588 T with Risk of Polycystic Ovary Syndrome in Chinese Women.  |  Yang, C., et al. 2022. Reprod Sci. 29: 1790-1800. PMID: 34642912
8. NaAsO2 decreases GSH synthesis by inhibiting GCLC and induces apoptosis through Hela cell mitochondrial damage, mediating the activation of the NF-κB/miR-21 signaling pathway.  |  Ran, S., et al. 2022. Ecotoxicol Environ Saf. 234: 113380. PMID: 35298964
9. Polymorphisms of the GCLC Gene Are Novel Genetic Markers for Susceptibility to Psoriasis Associated with Alcohol Abuse and Cigarette Smoking.  |  Efanova, E., et al. 2023. Life (Basel). 13: PMID: 37374099
10. Proximal 5'-flanking sequence of the human gamma-glutamylcysteine synthetase heavy subunit gene is involved in cisplatin-induced transcriptional up-regulation in a lung cancer cell line SBC-3.  |  Tomonari, A., et al. 1997. Biochem Biophys Res Commun. 236: 616-21. PMID: 9245699

The preferred γ-GCSc antibody is γ-GCSc Antibody (H-5): sc-390811.