γ Enolase Antibody (D-7): sc-376375

γ Enolase Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects γ Enolase in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). γ Enolase Antibody (D-7) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. γ Enolase is one of three isoenzymes of enolase, which are highly conserved cytoplasmic glycolytic enzymes crucial for energy metabolism. Specifically, γ Enolase is predominantly expressed in nervous tissue, where γ Enolase plays a vital role in the glycolytic pathway by catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate, a key step in energy production. γ Enolase expression in the nervous system is particularly important as γ Enolase may be involved in neuronal differentiation and function, highlighting potential roles in neurodevelopmental processes and neurodegenerative diseases. Understanding γ Enolase expression and function can provide insights into metabolic regulation in the brain and implications in various neurological conditions.

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γ Enolase Antibody (D-7) References:

1. Small cell carcinoma of urinary bladder is differentiated from urothelial carcinoma by chromogranin expression, absence of CD44 variant 6 expression, a unique pattern of cytokeratin expression, and more intense gamma-enolase expression.  |  Iczkowski, KA., et al. 1999. Histopathology. 35: 150-6. PMID: 10460660
2. An immunobioluminescence assay for gamma-gamma enolase activity in human serum and cerebrospinal fluid.  |  Wevers, RA., et al. 1988. Clin Chim Acta. 178: 141-50. PMID: 3072116
3. Creatine kinase BB and neuron-specific gamma-enolase are enhanced in leiomyosarcoma.  |  Usui, A., et al. 1988. Clin Chim Acta. 175: 109-11. PMID: 3168280
4. Preparation and purification of gamma gamma enolase (neuron-specific enolase) using high performance anion exchange chromatography.  |  Viallard, JL., et al. 1988. Neurochem Res. 13: 31-5. PMID: 3368027
5. Properties and application to immunoassay of monoclonal antibodies to neuron-specific gamma gamma enolase.  |  Kimura, S., et al. 1984. Biochim Biophys Acta. 799: 252-9. PMID: 6375733
6. Hybridization and isolation of human-rat beta gamma enolase.  |  Shimizu, A. and Kato, K. 1984. Biochim Biophys Acta. 786: 118-20. PMID: 6712954
7. Synapse formation is related to the onset of neuron-specific enolase immunoreactivity in the avian auditory and vestibular systems.  |  Whitehead, MC., et al. 1982. Dev Neurosci. 5: 298-307. PMID: 7140580
8. DNA sequences encoding enolase are remarkably conserved from yeast to mammals.  |  Verma, M. and Dutta, SK. 1994. Life Sci. 55: 893-9. PMID: 7520111
9. Coexpression of alpha and gamma enolase genes in neurons of adult rat brain.  |  Keller, A., et al. 1994. J Neurosci Res. 38: 493-504. PMID: 7815469
10. A comparative study of the distribution of alpha- and gamma-enolase subunits in cultured rat neural cells and fibroblasts.  |  Deloulme, JC., et al. 1997. Int J Dev Neurosci. 15: 183-94. PMID: 9178037
11. Expression of two neuronal markers, growth-associated protein 43 and neuron-specific enolase, in rat glial cells.  |  Sensenbrenner, M., et al. 1997. J Mol Med (Berl). 75: 653-63. PMID: 9351704
12. Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution.  |  Zhang, E., et al. 1997. Biochemistry. 36: 12526-34. PMID: 9376357

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γ Enolase Antibody (D-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-γ Enolase Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γ Enolase Antibody (D-7) Specifications

Alternate Product Name: γ Enolase

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 41-73

Epitope Target: γ Enolase

Epitope Species: human

Product Reactivity/Detection: γ Enolase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for γ Enolase Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin